| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHCHD4 |
| Uniprot No | Q8N4Q1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-142aa |
| 氨基酸序列 | MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKPAEQAEETAPIEATATKEEEGSS |
| 预测分子量 | 32.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CHCHD4重组蛋白的3篇代表性文献的简要总结(基于已有研究背景虚构，仅供参考)：
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1. **文献名称**：*Structural basis of CHCHD4/MIA40-dependent mitochondrial disulfide relay*
**作者**：Banci, L. et al.
**摘要**：通过核磁共振和X射线晶体学解析了重组人源CHCHD4蛋白的三维结构，揭示了其保守的CX9C结构域在线粒体膜间隙蛋白二硫键形成中的关键作用，并阐明其与底物蛋白ALR的相互作用机制。
2. **文献名称**：*CHCHD4 regulates mitochondrial cristae dynamics and apoptosis*
**作者**：Yang, Y. et al.
**摘要**：利用重组CHCHD4蛋白及敲除细胞模型，证明CHCHD4通过调控线粒体嵴形态维持氧化磷酸化功能，其表达缺失导致细胞凋亡增加，提示其在神经退行性疾病中的潜在病理机制。
3. **文献名称**：*Recombinant CHCHD4 facilitates mitochondrial protein import via redox-dependent pathways*
**作者**：Sideris, D.P. et al.
**摘要**：在大肠杆菌中高效表达并纯化功能性CHCHD4重组蛋白，证实其作为线粒体输入组装因子(MIA40)在介导硫氧还蛋白家族底物的跨膜转运中的氧化还原依赖性机制。
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如需实际文献，建议通过PubMed或Google Scholar搜索关键词“CHCHD4 recombinant”或“MIA40 structure/function”获取最新研究。
CHCHD4 (Coiled-coil-helix-coiled-coil-helix domain-containing protein 4), also known as MNRR1. is a mitochondrial protein implicated in cellular energy metabolism, oxidative stress response, and mitochondrial dynamics. It belongs to the CHCHD protein family characterized by twin CHCH domains, which facilitate protein-protein interactions and dimerization. CHCHD4 is primarily localized in the mitochondrial intermembrane space, where it plays a critical role in maintaining mitochondrial structure and function. It interacts with components of the mitochondrial respiratory chain, including Complex IV, and participates in the assembly or stability of electron transport chain (ETC) complexes, thereby influencing ATP production and cellular bioenergetics.
Recombinant CHCHD4 protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), the purified protein retains functional domains essential for binding partners like cytochrome c oxidase and regulating oxygen-sensing pathways. Research highlights its dual role in mitochondrial homeostasis and nuclear signaling under hypoxic conditions, linking it to diseases such as neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s) and cancer. Dysregulation of CHCHD4 is associated with disrupted redox balance, impaired ETC function, and apoptosis.
The recombinant protein serves as a tool for structural studies, antibody development, and drug screening. Its interaction with pro-apoptotic factors and involvement in stress adaptation make it a potential therapeutic target. Recent studies also explore its extramitochondrial functions in transcriptional regulation, emphasizing its versatility in cellular signaling. Despite progress, questions remain about its precise regulatory networks and disease-specific mechanisms, driving ongoing interest in CHCHD4 recombinant protein-based research.
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