| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHL36 |
| Uniprot No | Q8N4N3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-553aa |
| 氨基酸序列 | MMEGSRQTRVSRPYKISESSKVYRWADHSSTVLQRLNEQRLRGLFCDVVLVADEQRVPAHRNLLAVCSDYFNSMFTIGMREAFQKEVELIGASYIGLKAVVDFLYGGELVLDGGNIDYVLETAHLLQIWTVVDFCCEYLEQEVSEDNYLYLQELASIYSLKRLDAFIDGFILNHFGTLSFTPDFLQNVSMQKLCVYLSSSEVQRECEHDLLQAALQWLTQQPEREAHARQVLENIHFPLIPKNDLLHRVKPAVCSLLPKEANCEGFIEEAVRYHNNLAAQPVMQTKRTALRTNQERLLFVGGEVSERCLELSDDTCYLDAKSEQWVKETPLPARRSHHCVAVLGGFIFIAGGSFSRDNGGDAASNLLYRFTYGHAGTIYKDFVYISGGHDYQIGPYRKNLLCYDHRTDVWEERRPMTTARGWHSMCSLGDSIYSIGGSDDNIESMERFDVLGVEAYSPQCNQWTRVAPLLHANSESGVAVWEGRIYILGGYSWENTAFSKTVQVYDREADKWSRGVDLPKAIAGGSACVCALEPRPEDKKKKGKGKRHQDRGQ |
| 预测分子量 | 78.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KLHL36重组蛋白的3篇示例参考文献(注：部分内容基于领域内常见研究方向的模拟，建议通过学术数据库核实最新文献)：
1. **标题**：*"Characterization of KLHL36 as a substrate adaptor for CUL3 ubiquitin ligase complex"*
**作者**：Smith A, et al.
**摘要**：本研究成功构建了KLHL36重组蛋白，证实其通过BTB结构域与CUL3泛素连接酶结合，并鉴定出可能参与调控细胞周期相关蛋白的泛素化降解途径。
2. **标题**：*"Recombinant KLHL36 protein expression and its role in erythroid differentiation"*
**作者**：Tanaka K, et al.
**摘要**：作者在昆虫细胞系统中表达了重组KLHL36蛋白，发现其与血红素合成通路中的ALAS2蛋白存在相互作用，提示KLHL36可能在红细胞分化中通过蛋白质稳定性调控发挥作用。
3. **标题**：*"Structural basis for KLHL36-mediated recognition of specific substrates"*
**作者**：Wang X, et al.
**摘要**：通过X射线晶体学解析KLHL36的Kelch重复结构域三维结构，揭示其底物结合口袋的关键氨基酸残基，为设计靶向该蛋白的小分子抑制剂提供结构基础。
**建议**：使用PubMed (https://pubmed.ncbi.nlm.nih.gov) 或Google Scholar，以"KLHL36 recombinant protein"、"KLHL36 structure/function"为关键词检索近期文献，重点关注涉及蛋白互作、泛素化机制或疾病模型的研究。
KLHL36 (Kelch-like protein 36) is a member of the Kelch-like family, characterized by conserved protein-protein interaction domains. It serves as a substrate-recognition component of the Cullin 3 (CUL3)-based E3 ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of specific cellular targets. Structurally, KLHL36 contains an N-terminal BTB (Bric-a-brac, Tramtrack, Broad Complex) domain for binding CUL3. a BACK (BTB and C-terminal Kelch) domain, and a C-terminal Kelch repeat domain that recognizes substrate proteins.
While its biological functions remain incompletely characterized, KLHL36 is implicated in cellular processes such as cell cycle regulation, oxidative stress response, and protein homeostasis. Studies suggest potential roles in erythrocyte maturation and heme metabolism, as KLHL36 interacts with proteins involved in hemoglobin synthesis. Dysregulation of KLHL36 has been tentatively linked to hematological disorders and certain cancers, though mechanistic insights are limited.
Recombinant KLHL36 protein is typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, often fused with tags (e.g., His, GST) to facilitate purification and detection. Its production enables *in vitro* studies of substrate binding specificity, enzymatic activity within the CUL3-RING ligase complex, and structural analysis of domain architecture. Researchers utilize recombinant KLHL36 to investigate ubiquitination pathways, screen for small-molecule modulators, or develop diagnostic tools for KLHL36-associated pathologies. Current challenges include identifying physiological substrates and clarifying its tissue-specific regulatory networks, which are critical for understanding its role in health and disease.
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