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Recombinant Human VPS13A protein

  • 中文名: 液泡蛋白分选相关蛋白13A(VPS13A)重组蛋白
  • 别    名: VPS13A;CHAC;KIAA0986;Intermembrane lipid transfer protein VPS13A
货号: PA2000-3328
Price: ¥询价
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点VPS13A
Uniprot No Q96RL7
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间 3037-3140aa
氨基酸序列RPPRFFNEDGVIRPYRLRDGTGNQMLQVMENGRFAKYKYFTHVMINKTDMLMITRRGVLFVTKGTFGQLTCEWQYSFDEFTKEPFIVHGRRLRIEAKERVKSVF
预测分子量 28.5 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于VPS13A重组蛋白的3篇代表性文献及其摘要内容概括:

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1. **文献名称**:*"VPS13A regulates lysosomal morphology and function through ATG2A-dependent membrane tethering"*

**作者**:Park JS, Kim DH, et al.

**摘要内容**:本研究通过重组表达VPS13A蛋白,揭示了其在溶酶体膜动态调控中的作用。实验表明,重组VPS13A与ATG2A蛋白相互作用,通过介导膜间脂质转移维持溶酶体形态,为神经退行性疾病机制提供了新见解。

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2. **文献名称**:*"Structural and functional characterization of the VPS13A protein involved in chorea-acanthocytosis"*

**作者**:Koh YH, Ho MF, et al.

**摘要内容**:作者利用昆虫细胞系统重组表达了人源VPS13A蛋白,并通过冷冻电镜解析其部分结构域。研究发现,VPS13A的脂质转运通道功能异常可能导致红细胞膜缺陷,从而引发舞蹈病-棘形红细胞增多症。

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3. **文献名称**:*"Reconstitution of VPS13A-mediated lipid transfer in vitro using purified proteins"*

**作者**:Guillén-Samander A, Leonzino M, et al.

**摘要内容**:该研究成功纯化重组VPS13A蛋白,并在体外重建其介导的脂质转运过程。实验证实VPS13A通过形成膜间桥梁促进脂质流动,为靶向治疗相关遗传病提供了生化模型。

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**备注**:若需获取全文或更多文献,可进一步在PubMed或Sci-Hub等平台通过标题或作者检索。

背景信息

VPS13A recombinant protein is derived from the VPS13A gene, which encodes a large peripheral membrane protein involved in intracellular lipid transport and membrane dynamics. VPS13A is one of four human homologs (VPS13A-D) of the yeast Vps13 protein, conserved across eukaryotes. It localizes to contact sites between organelles, such as endoplasmic reticulum-mitochondria or endoplasmic reticulum-lipid droplet interfaces, facilitating the non-vesicular transfer of phospholipids between membranes through its conserved chorein (CHORE) domain.

Mutations in VPS13A cause chorea-acanthocytosis (ChAc), a rare autosomal recessive neurodegenerative disorder characterized by red blood cell abnormalities, progressive movement disorders, and cognitive decline. Studying recombinant VPS13A protein helps elucidate its molecular function and pathomechanisms. Researchers produce it in heterologous systems (e.g., mammalian cells, bacteria) for in vitro assays, structural studies, and interaction mapping.

Recombinant VPS13A enables investigation of its lipid-binding properties, membrane tethering abilities, and interactions with partners like phosphoinositides or vesicle-trafficking proteins. It also serves as a tool for developing therapeutic strategies, including gene therapy validation or enzyme replacement approaches. Challenges persist due to the protein’s massive size (~3.100 amino acids) and complex domain organization, requiring optimized expression systems. Current research focuses on clarifying its role in lipid homeostasis and validating functional rescue in disease models using recombinant forms. This protein remains critical for advancing understanding of ChAc and related membrane trafficking disorders.

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