| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | MSRB7 |
| Uniprot No | Q8VY86 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-144aa |
| 氨基酸序列 | MAAMTAAAVPATGSFQKQDEEWRAVLSPEQFRVLRLKGTDKRGKGEFTKKFEEGTYSCAGCGTALYKSTTKFDSGCGWPAFFDAIPGAIKQTPEAGGRRMEITCAVCDGHLGHVFKGEGYSTPTDQRHCVNSVSLKFSSAGSSQ |
| 预测分子量 | 31.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MSRB7重组蛋白的3篇代表性文献摘要(注：部分文献为示例性描述，实际引用时请核实具体来源)：
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1. **文献名称**：*Arabidopsis methionine sulfoxide reductase B7 interacts with glutathione peroxidase 3 to counteract oxidative stress*
**作者**：Li et al. (2018)
**摘要**：本研究通过大肠杆菌表达系统纯化了重组AtMSRB7蛋白，发现其与谷胱甘肽过氧化物酶GPX3存在物理互作。实验表明，重组MSRB7可修复氧化损伤的线粒体蛋白质，并与GPX3协同增强植物对氧化胁迫的耐受性，揭示了其在ROS清除中的协同机制。
2. **文献名称**：*Heterologous expression and functional analysis of rice MSRB7 in abiotic stress tolerance*
**作者**：Zhang et al. (2020)
**摘要**：研究者将水稻OsMSRB7基因克隆至原核表达系统，成功获得高纯度重组蛋白。酶活分析显示其对甲硫氨酸亚砜具有高效还原能力。转基因实验证实，重组OsMSRB7通过保护光系统II蛋白的完整性，显著提高宿主细胞对盐胁迫和干旱的抗性。
3. **文献名称**：*Structural insights into the catalytic mechanism of MSRB7 from Chlamydomonas reinhardtii*
**作者**：Wang et al. (2021)
**摘要**：本文解析了莱茵衣藻CrMSRB7重组蛋白的晶体结构，揭示了其活性位点的关键半胱氨酸残基。通过体外酶动力学实验，发现其优先还原游离型甲硫氨酸亚砜，且在光照胁迫下通过硫氧还蛋白系统维持酶活性，为设计抗氧化工程酶提供了结构基础。
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**注意**：以上文献为示例，实际研究中请通过学术数据库(如PubMed、Web of Science)检索具体文献，并核对作者与发表年份的准确性。
**Background of MSRB7 Recombinant Protein**
Methionine sulfoxide reductase B7 (MSRB7) is an enzyme belonging to the methionine sulfoxide reductase (MSR) family, which plays a critical role in mitigating oxidative damage in proteins. Oxidative stress leads to the oxidation of methionine residues, forming methionine sulfoxide, which can impair protein function. MSRs catalyze the reduction of methionine sulfoxide back to methionine, thereby repairing damaged proteins and maintaining cellular homeostasis. MSRB7 specifically targets the R-epimer of methionine sulfoxide and is distinguished by its unique substrate specificity and subcellular localization.
In plants, MSRB7 is predominantly localized to chloroplasts and is implicated in protecting photosynthetic machinery from reactive oxygen species (ROS) generated during light stress. Studies in *Arabidopsis thaliana* have shown that MSRB7 is vital for maintaining chloroplast function, stress resilience, and overall plant viability under adverse environmental conditions. Its recombinant form is engineered for research to explore its enzymatic mechanisms, structural features, and potential biotechnological applications.
Recombinant MSRB7 protein is typically produced using heterologous expression systems such as *E. coli* or yeast, enabling large-scale purification for functional studies. Structural analyses reveal conserved catalytic domains and a zinc-binding motif essential for its reductase activity. Beyond plant biology, MSRB7 has garnered interest in biomedical research due to its relevance in aging, neurodegenerative diseases, and pathologies linked to oxidative stress.
Overall, MSRB7 recombinant protein serves as a valuable tool for dissecting redox-regulatory pathways and developing strategies to enhance stress tolerance in crops or therapeutic interventions for oxidative damage-related disorders.
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