| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIGX |
| Uniprot No | Q8TBF5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 42-230aa |
| 氨基酸序列 | MCSEIILRQEVLKDGFHRDLLIKVKFGESIEDLHTCRLLIKQDIPAGLYVDPYELASLRERNITEAVMVSENFDIEAPNYLSKESEVLIYARRDSQCIDCFQAFLPVHCRYHRPHSEDGEASIVVNNPDLLMFCDQEFPILKCWAHSEVAAPCALENEDICQWNKMKYKSVYKNVILQVPVGLTVHTSL |
| 预测分子量 | 37.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的3篇关于PIGX重组蛋白的参考文献(注：实际文献需通过学术数据库检索验证)：
1. **文献名称**: "Functional characterization of PIGX in glycosylphosphatidylinositol biosynthesis"
**作者**: Hiroshi Ashida et al.
**摘要**: 本研究首次在大肠杆菌系统中重组表达了PIGX蛋白，证实其作为GPI锚定合成途径中的关键酶，通过体外实验揭示了PIGX与PIGY形成复合物催化N-乙酰葡糖胺转移的分子机制。
2. **文献名称**: "Recombinant PIGX expression optimization and structural analysis"
**作者**: Li W, Zhang Q.
**摘要**: 报道了利用昆虫杆状病毒系统高效表达可溶性PIGX重组蛋白的工艺，通过冷冻电镜解析了其三维结构，发现C端结构域对底物识别至关重要，为GPI相关疾病的药物开发提供结构基础。
3. **文献名称**: "PIGX deficiency disrupts protein trafficking in mammalian cells"
**作者**: Kinoshita T, Fujita M.
**摘要**: 通过构建PIGX基因敲除细胞系并回补重组蛋白，证明PIGX缺失导致GPI锚定蛋白无法正确膜定位，引发内质网应激反应，揭示了其在真核细胞分泌通路中的质量控制作用。
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提示：以上文献信息为模拟生成，真实研究需通过PubMed/Google Scholar检索关键词"PIGX recombinant"、"PIGX GPI biosynthesis"等获取。建议结合具体研究方向筛选近年高被引论文。
**Background of PIGX Recombinant Protein**
PIGX recombinant protein is a biologically engineered molecule designed for applications in biomedical research, diagnostics, and therapeutic development. Derived from advanced recombinant DNA technology, it is typically expressed in heterologous systems such as *E. coli*, yeast, or mammalian cell cultures (e.g., CHO cells) to ensure high purity, specificity, and scalability. The "PIGX" designation often refers to its origin or functional association with specific pathogens or physiological processes. For instance, in veterinary and zoonotic disease research, PIGX may be linked to antigenic proteins from pathogens like porcine viruses (e.g., porcine circovirus or influenza), serving as a critical tool for antibody production, vaccine development, or serological assays.
The development of PIGX recombinant protein is driven by the need for reliable, standardized reagents to study host-pathogen interactions, immune responses, or molecular mechanisms. Unlike native proteins extracted from pathogens, recombinant versions eliminate biosafety risks while offering consistency in batch-to-batch performance. Its production often involves codon optimization for enhanced expression, affinity tags (e.g., His-tag) for simplified purification, and validation via techniques like SDS-PAGE, Western blot, or ELISA to confirm structural integrity and bioactivity.
In practical applications, PIGX recombinant protein is widely utilized in diagnostic kits to detect antibodies in animal or human sera, aiding in disease surveillance. It also serves as a candidate antigen for subunit vaccines, leveraging its ability to elicit targeted immune responses. Additionally, in basic research, it facilitates the study of protein-protein interactions, signaling pathways, or structural biology.
Overall, PIGX recombinant protein exemplifies the synergy between genetic engineering and translational science, offering a versatile, safe, and cost-effective solution for addressing challenges in both human and veterinary medicine.
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