| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TXNDC5 |
| Uniprot No | Q8NBS9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-324aa |
| 氨基酸序列 | MEDAKVYVAKVDCTAHSDVCSAQGVRGYPTLKLFKPGQEAVKYQGPRDFQTLENWMLQTLNEEPVTPEPEVEPPSAPELKQGLYELSASNFELHVAQGDHFIKFFAPWCGHCKALAPTWEQLALGLEHSETVKIGKVDCTQHYELCSGNQVRGYPTLLWFRDGKKVDQYKGKRDLESLREYVESQLQRTETGATETVTPSEAPVLAAEPEADKGTVLALTENNFDDTIAEGITFIKFYAPWCGHCKTLAPTWEELSKKEFPGLAGVKIAEVDCTAERNICSKYSVRGYPTLLLFRGGKKVSEHSGGRDLDSLHRFVLSQAKDEL |
| 预测分子量 | 63.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TXNDC5重组蛋白的模拟参考文献示例(文献内容为假设性概括，实际引用时请核实真实文献)：
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1. **标题**：*Recombinant TXNDC5 Enhances Endoplasmic Reticulum Stress Resistance in Hepatocytes*
**作者**：Chen L, et al.
**摘要**：研究利用大肠杆菌系统表达并纯化重组TXNDC5蛋白，发现其通过调节内质网应激相关蛋白(如CHOP和ATF4)显著提高肝细胞对氧化应激的抵抗能力，提示其在代谢性疾病中的潜在作用。
2. **标题**：*TXNDC5 Recombinant Protein Promotes Collagen Synthesis in Cardiac Fibroblasts via TGF-β1 Signaling*
**作者**：Kim S, et al.
**摘要**：通过体外实验证实重组TXNDC5蛋白通过激活TGF-β1/Smad通路，促进心脏成纤维细胞的胶原沉积，为心脏纤维化机制提供了新靶点。
3. **标题**：*Structural Characterization of Human TXNDC5 Reveals a Novel Disulfide Isomerase Activity*
**作者**：Gupta R, et al.
**摘要**：报道了重组TXNDC5蛋白的晶体结构解析，发现其具有独特的二硫键异构酶活性，并验证了其在内质网蛋白折叠中的功能。
4. **标题**：*Recombinant TXNDC5 as a Therapeutic Target in Rheumatoid Arthritis: Suppression of Synovial Inflammation*
**作者**：Müller P, et al.
**摘要**：实验表明，抑制重组TXNDC5蛋白的活性可减少类风湿关节炎滑膜细胞中炎症因子(如IL-6、TNF-α)的释放，提示其作为治疗靶点的潜力。
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**注意**：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献并核对作者、标题及摘要信息。
**Background of TXNDC5 Recombinant Protein**
TXNDC5 (Thioredoxin Domain-Containing Protein 5) is a member of the thioredoxin (TRX) protein family, characterized by its conserved thioredoxin-like domain. This protein plays a role in redox regulation, primarily through its disulfide isomerase activity, which assists in protein folding and oxidative stress mitigation. TXNDC5 is localized in the endoplasmic reticulum (ER), where it participates in ER-associated degradation (ERAD) and the unfolded protein response (UPR), critical for maintaining cellular homeostasis under stress conditions.
Structurally, recombinant TXNDC5 is produced using expression systems like *E. coli* or mammalian cells, enabling studies on its biochemical properties and interactions. Its recombinant form retains the active thioredoxin domain, facilitating research into its enzymatic mechanisms. TXNDC5 has been implicated in various pathologies, including cardiovascular diseases, cancer, and autoimmune disorders. For instance, it is overexpressed in endothelial cells during atherosclerosis, promoting inflammation and plaque instability. In cancer, TXNDC5 supports tumor survival by enhancing ER stress adaptation and evading apoptosis.
Research on recombinant TXNDC5 also explores its role in post-translational modifications, such as mediating disulfide bond formation in secreted or membrane-bound proteins. Additionally, its interaction with chaperones like GRP78 highlights its involvement in ER quality control.
The development of TXNDC5 recombinant protein has advanced therapeutic and diagnostic applications. Inhibitors targeting TXNDC5 are under investigation for diseases linked to ER stress and oxidative damage. Overall, TXNDC5 serves as a key molecular player in cellular stress responses, with recombinant variants providing essential tools for mechanistic and translational studies.
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