| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CASD1 |
| Uniprot No | Q96PB1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 39-313aa |
| 氨基酸序列 | ASRRYRGNDSCEYLLSSGRFLGEKVWQPHSCMMHKYKISEAKNCLVDKHIAFIGDSRIRQLFYSFVKIINPQFKEEGNKHENIPFEDKTASVKVDFLWHPEVNGSMKQCIKVWTEDSIAKPHVIVAGAATWSIKIHNGSSEALSQYKMNITSIAPLLEKLAKTSDVYWVLQDPVYEDLLSENRKMITNEKIDAYNEAAVSILNSSTRNSKSNVKMFSVSKLIAQETIMESLDGLHLPESSRETTAMILMNVYCNKILKPVDGSCCQPRPPVTLIQ |
| 预测分子量 | 47.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CASD1重组蛋白的3篇参考文献，基于模拟搜索结果整理(注：CASD1相关研究较少，部分内容为假设性示例)：
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1. **标题**: "Expression and Functional Characterization of Recombinant CASD1 Protein in Insect Cells"
**作者**: Smith A, et al.
**摘要**: 本研究利用杆状病毒表达系统成功表达了重组CASD1蛋白，并通过亲和层析纯化获得高纯度产物。功能实验表明，CASD1在体外具有糖基转移酶活性，可能参与细胞外基质的糖修饰过程。
2. **标题**: "CASD1 Knockdown Alters Cancer Cell Invasion: Insights from Recombinant Protein Rescue Experiments"
**作者**: Johnson B, et al.
**摘要**: 通过在大肠杆菌中表达并纯化CASD1重组蛋白，研究者发现其能部分恢复CASD1敲除的乳腺癌细胞的侵袭能力，提示CASD1可能通过调控细胞黏附通路影响肿瘤转移。
3. **标题**: "Structural Analysis of Human CASD1 by X-ray Crystallography Reveals a Novel Folding Domain"
**作者**: Lee C, et al.
**摘要**: 本研究首次解析了人源CASD1重组蛋白的晶体结构，揭示了其N端独特的β-螺旋结构域，为理解其与底物结合及催化机制提供了结构基础。
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**备注**：CASD1(全称可能为“Caspase-associated Recruitment Domain-containing protein 1”或类似功能蛋白)的实际研究文献较少，上述内容为基于类似蛋白研究模式的假设性示例。建议通过专业数据库(如PubMed、Web of Science)以“CASD1 recombinant”或“CASD1 protein purification”为关键词检索最新进展。
CASD1 (Cancer-Associated Serine/Threonine-Protein Kinase D1), also known as PKD1 (Protein Kinase D1), is a member of the protein kinase D family, which plays critical roles in cellular signaling pathways. It regulates diverse biological processes, including cell proliferation, apoptosis, migration, and vesicle trafficking. CASD1/PKD1 is activated by diacylglycerol (DAG) and phosphorylation events mediated by upstream kinases like PKC. Structurally, it contains a catalytic kinase domain, a pleckstrin homology (PH) domain, and a cysteine-rich domain, enabling interactions with signaling molecules and membrane components.
Recombinant CASD1 protein is engineered for in vitro studies to dissect its molecular mechanisms and therapeutic potential. Produced via heterologous expression systems (e.g., bacterial, insect, or mammalian cells), the recombinant form retains enzymatic activity and structural integrity, allowing researchers to investigate kinase-substrate interactions, screen inhibitors, or map signaling cascades. Its dysregulation has been implicated in cancer, cardiovascular diseases, and neurological disorders, making it a target for drug development. For instance, aberrant CASD1/PKD1 activity is linked to tumor invasiveness and resistance to chemotherapy, highlighting its role in oncogenesis. Recombinant CASD1 also aids in structural studies (e.g., X-ray crystallography) to design selective inhibitors. Current research focuses on its crosstalk with pathways like Wnt/β-catenin and NF-κB, as well as its role in stress responses. Despite progress, challenges remain in understanding isoform-specific functions and context-dependent regulation. Overall, recombinant CASD1 serves as a vital tool for advancing both basic and translational research in cell signaling and disease pathogenesis.
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