| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB3C |
| Uniprot No | Q96E17 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-227aa |
| 氨基酸序列 | MRHEAPMQMASAQDARYGQKDSSDQNFDYMFKLLIIGNSSVGKTSFLFRYADDSFTSAFVSTVGIDFKVKTVFKNEKRIKLQIWDTAGQERYRTITTAYYRGAMGFILMYDITNEESFNAVQDWSTQIKTYSWDNAQVILVGNKCDMEDERVISTERGQHLGEQLGFEFFETSAKDNINVKQTFERLVDIICDKMSESLETDPAITAAKQNTRLKETPPPPQPNCAC |
| 预测分子量 | 42.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB3C重组蛋白的3篇参考文献示例(注：以下为模拟内容，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: "Expression and Functional Characterization of Recombinant RAB3C GTPase in Cancer Cell Secretion"
**作者**: Li, Y., et al.
**摘要**: 该研究通过大肠杆菌系统成功表达并纯化重组RAB3C蛋白，验证其GTPase活性，并发现其在乳腺癌细胞外泌体分泌中起调控作用，促进肿瘤微环境的重塑。
2. **文献名称**: "RAB3C Structural Dynamics Revealed by Cryo-EM and Recombinant Protein Mutagenesis"
**作者**: Martinez, J.P., et al.
**摘要**: 利用重组RAB3C蛋白进行冷冻电镜结构解析，揭示了其与GTP/GDP结合时的构象差异，并鉴定出关键氨基酸残基对GTP水解活性的影响。
3. **文献名称**: "RAB3C Knockdown Impairs Insulin Secretion via Disrupting Vesicle Trafficking in Pancreatic β-cells"
**作者**: Chen, H., et al.
**摘要**: 通过体外重组RAB3C蛋白与胰岛β细胞囊泡共孵育实验，证明RAB3C通过调控囊泡锚定步骤影响胰岛素分泌，为糖尿病机制研究提供新靶点。
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**提示**：实际文献需通过PubMed、Google Scholar等平台检索关键词“RAB3C recombinant protein expression/function/structure”获取。建议结合研究领域(如肿瘤、神经或分泌通路)进一步筛选。
**Background of RAB3C Recombinant Protein**
RAB3C is a member of the RAB GTPase family, a group of small GTP-binding proteins critical for regulating vesicle trafficking, membrane fusion, and exocytosis in eukaryotic cells. Specifically, RAB3C belongs to the RAB3 subfamily, which includes four isoforms (RAB3A-D) implicated in secretory processes, particularly neurotransmitter release and hormone secretion. While RAB3A has been extensively studied in synaptic vesicle dynamics, RAB3C shares structural homology but exhibits distinct expression patterns and functional roles, primarily in non-neuronal tissues such as endocrine cells, immune cells, and certain cancers.
Recombinant RAB3C protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional RAB3C for research. This engineered protein often includes tags (e.g., GST, His-tag) for ease of purification and detection. Its study focuses on elucidating RAB3C’s interactions with effector proteins, such as Rabphilin-3A or Munc-18. and its regulatory role in vesicle docking, priming, and fusion via GTP-GDP cycling. Dysregulation of RAB3C has been linked to pathological conditions, including tumor progression, metastasis, and endocrine disorders, highlighting its potential as a therapeutic target or biomarker.
Current research leverages recombinant RAB3C to dissect its involvement in exocytosis pathways, cancer cell invasiveness, and metabolic signaling. Structural analyses (e.g., crystallography) and functional assays (e.g., GTPase activity, binding kinetics) using the recombinant protein provide insights into its molecular mechanisms. Despite overlapping functions with other RAB3 isoforms, RAB3C’s unique tissue specificity and post-translational modifications underscore its specialized role in cellular trafficking networks. Further studies aim to clarify its contributions to diseases and exploit its regulatory pathways for biomedical applications.
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