| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | sdrE |
| Uniprot No | Q932F7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 53-601aa |
| 氨基酸序列 | AENTSTENAKQDDATTSDNKEVVSETENNSTTENDSTNPIKKETNTDSQPEAKEESTTSSTQQQQNNVTATTETKPQNIEKENVKPSTDKTATEDTSVILEEKKAPNYTNNDVTTKPSTSEIQTKPTTPQESTNIENSQPQPTPSKVDNQVTDATNPKEPVNVSKEELKNNPEKLKELVRNDNNTDRSTKPVATAPTSVAPKRLNAKMRFAVAQPAAVASNNVNDLITVTKQTIKVGDGKDNVAAAHDGKDIEYDTEFTIDNKVKKGDTMTINYDKNVIPSDLTDKNDPIDITDPSGEVIAKGTFDKATKQITYTFTDYVDKYEDIKARLTLYSYIDKQAVPNETSLNLTFATAGKETSQNVSVDYQDPMVHGDSNIQSIFTKLDENKQTIEQQIYVNPLKKTATNTKVDIAGSQVDDYGNIKLGNGSTIIDQNTEIKVYKVNPNQQLPQSNRIYDFSQYEDVTSQFDNKKSFSNNVATLDFGDINSAYIIKVVSKYTPTSDGELDIAQGTSMRTTDKYGYYNYAGYSNFIVTSNDTGGGDGTVKPEEK |
| 预测分子量 | 64.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于sdrE重组蛋白的3篇示例参考文献，内容基于学术研究的常见方向虚构概括，仅供参考：
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1. **标题**: "Recombinant SdrE Protein Mediates Host Cell Adhesion in *Staphylococcus aureus* Infections"
**作者**: Smith A, et al.
**摘要**: 本研究成功表达并纯化了重组SdrE蛋白，证实其在金黄色葡萄球菌与宿主细胞(如内皮细胞)粘附中的关键作用，为靶向治疗提供了潜在靶点。
2. **标题**: "Structural and Functional Analysis of the SdrE Surface Protein via Recombinant Expression"
**作者**: Zhang Y, et al.
**摘要**: 通过大肠杆菌系统表达重组SdrE蛋白，利用X射线晶体学解析其三维结构，揭示了其独特的β-折叠构象和可能的免疫逃逸机制。
3. **标题**: "Evaluation of Recombinant SdrE as a Vaccine Candidate Against Staphylococcal Infections"
**作者**: Jones R, et al.
**摘要**: 在小鼠模型中评估重组SdrE蛋白的免疫原性，结果显示其能诱导特异性抗体反应，显著降低细菌载量，提示其作为疫苗组分的潜力。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词“sdrE recombinant protein Staphylococcus aureus”获取最新研究。
**Background of SdrE Recombinant Protein**
SdrE (Serine-aspartate repeat protein E) is a cell wall-anchored surface protein produced by *Staphylococcus aureus*, a Gram-positive pathogen associated with diverse infections, including bacteremia and endocarditis. It belongs to the Sdr family, a subgroup of microbial surface components recognizing adhesive matrix molecules (MSCRAMMs), which facilitate bacterial adherence to host tissues and immune evasion. SdrE is characterized by its conserved structural domains: an N-terminal signal peptide, an A-region mediating ligand binding, B-repeat sequences involved in immune interaction, and a C-terminal LPXTG motif for cell wall anchoring.
The recombinant SdrE protein is generated through genetic engineering, typically by cloning the *sdrE* gene into expression vectors (e.g., *E. coli*), followed by purification. This allows large-scale production of the protein for functional and immunological studies. Research highlights SdrE’s role in binding host extracellular matrix components, such as complement factor H, to inhibit opsonophagocytosis, aiding bacterial survival. It also interacts with platelets, contributing to thrombus formation in endocarditis.
Studies on recombinant SdrE focus on its potential as a diagnostic marker, vaccine candidate, or therapeutic target. Epitope mapping reveals immunogenic regions, supporting its use in antibody-based therapies. Additionally, structural analyses of the recombinant protein provide insights into mechanisms of pathogenicity and guide drug design. Despite progress, challenges remain in understanding its full regulatory network and cross-strain variability. Overall, SdrE recombinant protein serves as a vital tool in combating *S. aureus* infections through translational research.
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