| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLS1 |
| Uniprot No | Q96330 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-336aa |
| 氨基酸序列 | MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAVVKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQKDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDGLGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQVFKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREKIVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLPLD |
| 预测分子量 | 43.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FLS1重组蛋白的3篇虚构参考文献示例(基于常见研究方向模拟，实际文献需根据具体领域核实)：
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1. **文献名称**: *Heterologous Expression and Functional Characterization of Flavonol Synthase 1 (FLS1) in Arabidopsis*
**作者**: Zhang, Y., et al.
**摘要**: 本研究通过在大肠杆菌系统中重组表达拟南芥来源的FLS1蛋白，验证了其催化二氢黄酮醇转化为黄酮醇的关键酶活性，并优化了蛋白纯化条件，为植物代谢工程提供基础。
2. **文献名称**: *Structural Insights into FLS1 Catalytic Mechanism by X-ray Crystallography*
**作者**: Lee, S., & Kim, J.
**摘要**: 通过X射线晶体学解析了重组FLS1的三维结构，揭示了其底物结合口袋的关键氨基酸残基，阐明了该酶催化反应的空间构象变化机制。
3. **文献名称**: *FLS1 Recombinant Protein Attenuates Inflammation in Murine Models via Flavonoid Biosynthesis*
**作者**: Müller, R., et al.
**摘要**: 利用哺乳动物细胞表达系统制备功能性FLS1重组蛋白，证明其通过促进特定黄酮类化合物合成，显著减轻小鼠模型中的炎症反应，提示其潜在药用价值。
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注：以上为模拟示例，实际文献需通过PubMed、Web of Science等平台检索。若需真实文献，请提供FLS1蛋白的全称及研究领域。
**Background of FLS1 Recombinant Protein**
FLS1 (Flagellin Sensing 1) is a plant pattern recognition receptor (PRR) that plays a critical role in innate immunity. It is primarily known for its ability to detect bacterial flagellin, a key pathogen-associated molecular pattern (PAMP), and initiate immune responses against invading pathogens. FLS1 was first identified in *Arabidopsis thaliana* and belongs to the leucine-rich repeat (LRR) receptor-like kinase (RLK) family. Its extracellular LRR domain specifically recognizes a conserved 22-amino acid epitope (flg22) of bacterial flagellin, triggering downstream signaling cascades that enhance plant resistance.
The recombinant FLS1 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its structural and functional properties. This protein is pivotal in dissecting molecular mechanisms underlying plant-pathogen interactions, particularly how plants detect and respond to microbial threats. Research on FLS1 has revealed its role in activating defense responses, including reactive oxygen species (ROS) production, mitogen-activated protein kinase (MAPK) signaling, and transcriptional reprogramming of defense-related genes.
Beyond basic research, FLS1 recombinant protein has applications in agricultural biotechnology, such as developing pathogen-resistant crops or bioinspired immune-boosting agents. Its study also contributes to understanding receptor-ligand interactions and the evolution of plant immune systems. Recent advances in structural biology, including cryo-EM and X-ray crystallography, have provided insights into FLS1’s 3D conformation and binding dynamics with flg22. offering potential for designing synthetic immune receptors or novel antimicrobial strategies.
In summary, FLS1 recombinant protein serves as a key tool for exploring plant immunity and advancing sustainable crop protection solutions.
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