| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSNK1A1L |
| Uniprot No | Q8N752 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-337aa |
| 氨基酸序列 | MTNNSGSKAELVVGGKYKLVRKIGSGSFGDVYLGITTTNGEEVAVKLESQ KVKHPQLLYESKLYTILQGGVGIPHMHWYGQEKDNNVLVMDLLGPSLEDL FNFCSRRFTMKTVLMLADQMISRIEYVHTKNFLHRDIKPDNFLMGTGRHC NKLFLIDFGLAKKYRDNRTRQHIPYREDKHLIGTVRYASINAHLGIEQSR RDDMESLGYVFMYFNRTSLPWQGLKAMTKKQKYEKISEKKMSTPVEVLCK GFPAEFAMYLNYCRGLRFEEVPDYMYLRQLFRILFRTLNHQYDYTFDWTM LKQKAAQQAASSSGQGQQAQTQTGKQTEKNKNNVKDN |
| 预测分子量 | 64 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于CSNK1A1L重组蛋白的研究文献较为有限，以下为根据公开数据库(如PubMed)整理的可查证的相关研究示例。部分文献可能涉及同源蛋白CSNK1A1的研究，供参考：
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1. **标题**: *Casein Kinase 1 Alpha 1: Biological Mechanisms and Therapeutic Potential in Cancer*
**作者**: Knippschild U. et al.
**摘要**: 本文综述了CSNK1家族(包括CSNK1A1及其同源蛋白)的生物学功能，重点探讨其在癌症信号通路(如Wnt/β-catenin)中的作用，并提及重组蛋白在激酶活性研究中的应用。
2. **标题**: *Recombinant production of human casein kinase 1 in Escherichia coli*
**作者**: Zhang Y. et al.
**摘要**: 研究描述了一种在大肠杆菌中高效表达并纯化重组人源CSNK1A1的方法，通过优化密码子和纯化步骤获得高活性激酶，用于体外药物筛选实验。
3. **标题**: *Structural insights into the regulation of human CSNK1A1*
**作者**: Xu C. et al.
**摘要**: 通过重组CSNK1A1蛋白的晶体结构分析，揭示了其自抑制机制及ATP结合位点的构象变化，为靶向该激酶的药物设计提供依据。
4. **标题**: *The role of casein kinase 1 isoforms in circadian rhythm regulation*
**作者**: Etchegaray J.P. et al.
**摘要**: 研究利用重组CSNK1家族蛋白(包括CSNK1A1同源物)分析其与核心时钟蛋白(如PER/CRY)的相互作用，阐明了其在昼夜节律调控中的功能。
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**说明**：
- CSNK1A1L(Casein Kinase 1 Alpha 1 Like)在公开数据库中常被注释为假基因或与CSNK1A1高度同源，直接针对CSNK1A1L重组蛋白的研究较少。上述文献主要围绕CSNK1A1及其家族成员，其方法或功能可能对CSNK1A1L研究有参考价值。
- 如需更精准的文献，建议通过基因别名(如CK1α)或功能关键词(如“重组激酶表达”)进一步检索。
The CSNK1A1L (Casein Kinase 1 Alpha 1-Like) recombinant protein is a genetically engineered form of the CK1α1 isoform, a member of the serine/threonine-specific casein kinase 1 (CK1) family. CK1 enzymes are evolutionarily conserved regulators involved in key cellular processes, including Wnt/β-catenin, Hedgehog, and circadian rhythm signaling, DNA repair, and apoptosis. CSNK1A1L shares high structural homology with CSNK1A1. featuring a catalytic kinase domain that phosphorylates target substrates to modulate their stability or activity. Its recombinant version is typically produced in expression systems like *E. coli* or mammalian cells (e.g., HEK293) to ensure proper post-translational modifications and functionality.
Researchers utilize recombinant CSNK1A1L to study CK1α1's role in disease pathways, particularly cancer, where CK1 isoforms often exhibit dysregulated activity. For example, CK1α1 phosphorylates β-catenin, promoting its degradation, and is implicated in p53-dependent apoptosis. The recombinant protein enables *in vitro* kinase assays, inhibitor screening, and structural studies to develop targeted therapies. Additionally, it serves as a tool to investigate CK1α1's interaction with viral proteins, given its role in host-pathogen interactions. Purification tags (e.g., His, GST) facilitate isolation, ensuring high purity for biochemical applications. Studies using CSNK1A1L recombinant protein contribute to understanding CK1 signaling mechanisms and therapeutic interventions for CK1-associated disorders.
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