| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NMNAT3 |
| Uniprot No | Q96T66 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-252aa |
| 氨基酸序列 | MKSRIPVVLLACGSFNPITNMHLRMFEVARDHLHQTGMYQVIQGIISPVN DTYGKKDLAASHHRVAMARLALQTSDWIRVDPWESEQAQWMETVKVLRHH HSKLLRSPPQMEGPDHGKALFSTPAAVPELKLLCGADVLKTFQTPNLWKD AHIQEIVEKFGLVCVGRVGHDPKGYIAESPILRMHQHNIHLAKEPVQNEI SATYIRRALGQGQSVKYLIPDAVITYIKDHGLYTKGSTWKGKSTQSTEGK TS |
| 预测分子量 | 30 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于NMNAT3重组蛋白的参考文献摘要概括(虚构示例，供参考)：
1. **文献名称**：*Structural and functional characterization of recombinant human NMNAT3*
**作者**：Zhang Y. et al. (2020)
**摘要**：研究报道了通过大肠杆菌系统高效表达并纯化重组人源NMNAT3蛋白，解析其晶体结构，揭示其催化NAD+合成的关键活性位点及底物结合模式，证实其在线粒体中的酶活稳定性。
2. **文献名称**：*NMNAT3 recombinant protein mitigates mitochondrial dysfunction in age-related metabolic disorders*
**作者**：Lee S. et al. (2021)
**摘要**：利用哺乳动物细胞表达的重组NMNAT3蛋白，证明其通过提升细胞内NAD+水平改善线粒体能量代谢，并在高糖诱导的细胞模型中缓解氧化应激损伤，提示其在代谢疾病中的治疗潜力。
3. **文献名称**：*Comparative analysis of NMNAT isoforms: Enzymatic properties of recombinant NMNAT3 in neuronal protection*
**作者**：Johnson R. et al. (2019)
**摘要**：对比NMNAT1/2/3重组蛋白的酶动力学参数，发现NMNAT3对特定底物(如线粒体NADH)具有独特亲和力，并在体外神经元模型中展示出抑制轴突退化的活性，提示亚型特异性功能。
(注：以上内容为模拟生成，实际文献需通过数据库检索确认。)
NMNAT3 (Nicotinamide Mononucleotide Adenylyltransferase 3) is a member of the NMNAT enzyme family, which plays a critical role in cellular metabolism by catalyzing the synthesis of nicotinamide adenine dinucleotide (NAD+), an essential cofactor in redox reactions and signaling pathways. Unlike other isoforms (NMNAT1 and NMNAT2), NMNAT3 is uniquely localized to mitochondria in certain tissues, though its subcellular distribution may vary across species. This mitochondrial association suggests distinct roles in maintaining NAD+ homeostasis, energy production, and mitochondrial function, linking it to cellular processes such as aging, stress response, and neurodegeneration.
Recombinant NMNAT3 protein is produced through genetic engineering, typically by expressing the NMNAT3 gene in bacterial or mammalian cell systems, followed by purification to ensure high specificity and activity. Its recombinant form enables precise study of enzymatic mechanisms, structure-function relationships, and interactions with substrates or inhibitors. Researchers utilize NMNAT3 recombinant protein to explore its neuroprotective potential, as NAD+ depletion is implicated in neurodegenerative diseases like Alzheimer’s and Parkinson’s. Additionally, it serves as a tool to investigate mitochondrial dysfunction in metabolic disorders and aging-related pathologies.
Recent studies highlight NMNAT3’s dual enzymatic and chaperone-like functions, particularly in mitigating protein aggregation—a feature relevant to neurodegenerative conditions. However, its low abundance and tissue-specific expression pose challenges in characterizing endogenous functions. Recombinant NMNAT3 thus bridges gaps in understanding its biological significance while offering therapeutic avenues, such as NAD+ supplementation strategies. Ongoing research focuses on optimizing its stability, delivery mechanisms, and tissue targeting to harness its potential in treating mitochondrial diseases or age-associated decline.
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