| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FMR1NB |
| Uniprot No | Q8N0W7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 90-183aa |
| 氨基酸序列 | LCSGSSYFVLANGHILPNSENAHGQSLEEDSALEALLNFFFPTTCNLRENQVAKPCNELQDLSESECLRHKCCFSSSGTTSFKCFAPFRDVPKQ |
| 预测分子量 | 15.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FMR1NB重组蛋白的假设性参考文献示例(仅供参考，建议通过学术数据库验证具体文献)：
1. **标题**: *Expression and Functional Analysis of Recombinant FMR1NB in Neuronal Cells*
**作者**: Chen L, et al.
**摘要**: 研究利用哺乳动物表达系统成功制备FMR1NB重组蛋白，并发现其通过调控突触相关蛋白表达促进神经元突触形成。
2. **标题**: *FMR1NB Recombinant Protein Binds to RNA and Modulates Translational Activity in vitro*
**作者**: Wang R, et al.
**摘要**: 通过体外实验证实FMR1NB重组蛋白具有RNA结合能力，可能通过调节靶mRNA翻译影响神经发育疾病机制。
3. **标题**: *Crystallization and Structural Insights of FMR1NB Recombinant Protein*
**作者**: Martinez D, et al.
**摘要**: 首次解析FMR1NB重组蛋白的晶体结构，揭示其与FMR1蛋白的结构相似性及潜在功能关联。
4. **标题**: *Therapeutic Potential of FMR1NB Recombinant Protein in Fragile X Syndrome Models*
**作者**: Kim S, et al.
**摘要**: 在脆性X综合征小鼠模型中，外源性FMR1NB重组蛋白部分挽救了认知缺陷，提示其作为替代疗法的可能性。
**注意**：以上内容为基于领域知识的模拟文献，实际研究中需通过PubMed、Google Scholar等平台检索具体文献。若需精准结果，请提供更详细的背景信息或修正术语拼写。
The FMR1NB (FMR1 neighbor) gene, located adjacent to the fragile X mental retardation 1 (FMR1) gene on the X chromosome, encodes a protein with emerging roles in cellular processes and disease. While FMR1 is widely studied for its association with fragile X syndrome (FXS) due to CGG repeat expansions, FMR1NB has garnered interest as a neighboring gene with potential functional interplay. The FMR1NB protein shares structural motifs with FMRP (FMR1-encoded protein), including RNA-binding domains, suggesting involvement in mRNA regulation, though its exact mechanisms remain under investigation.
Recombinant FMR1NB protein is engineered using expression systems (e.g., bacterial, mammalian) to study its molecular interactions, post-translational modifications, and role in pathways linked to neurodevelopment or cancer. Interest in this protein stems from observations that FMR1NB expression may be dysregulated in FXS and certain cancers, hinting at compensatory mechanisms or independent pathological contributions. Researchers utilize recombinant FMR1NB to explore its binding partners, subcellular localization, and potential as a therapeutic target or biomarker.
Current studies focus on clarifying whether FMR1NB functionally overlaps with FMRP in synaptic plasticity or acts through distinct pathways. Challenges include elucidating its regulation under FMR1 silencing and its relevance in FXS comorbidities or oncogenesis. Recombinant tools are pivotal in dissecting these aspects, offering insights into both basic biology and translational applications for neurodevelopmental disorders and oncology.
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