| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OXNAD1 |
| Uniprot No | Q96HP4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-312aa |
| 氨基酸序列 | AIRIEAASLRLTLSTLRHLTLTSIMKSKRKTDHMERTASVLRREIVSAAKVCGAASESPSVKSLRLLVADQDFSFKAGQWVDFFIPGVSVVGGFSICSSPRLLEQERVIELAVKYTNHPPALWVHNTCTLDCEVAVRVGGEFFFDPQPADASRNLVLIAGGVGINPLLSILRHAADLLREQANKRNGYEIGTIKLFYSAKNTSELLFKKNILDLVNEFPEKIACSLHVTKQTTQINAELKPYITEGRITEKEIRDHISKETLFYICGPPPMTDFFSKQLENNHVPKEHICFEKWW |
| 预测分子量 | 49.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OXNAD1重组蛋白的3篇参考文献的简要信息：
1. **文献名称**: "Recombinant OXNAD1 protein regulates mitochondrial apoptosis via interaction with Bcl-2 family proteins"
**作者**: Zhang L, et al.
**摘要**: 该研究通过大肠杆菌表达系统成功制备了重组OXNAD1蛋白，并发现其通过与Bcl-2家族蛋白相互作用调控线粒体途径的细胞凋亡，为癌症治疗提供了潜在靶点。
2. **文献名称**: "Expression and functional characterization of OXNAD1 in hepatocellular carcinoma progression"
**作者**: Wang Y, et al.
**摘要**: 研究团队利用昆虫杆状病毒系统表达重组OXNAD1蛋白，证实其在肝癌细胞中通过抑制Wnt/β-catenin信号通路降低肿瘤侵袭性，提示其作为生物标志物的潜力。
3. **文献名称**: "OXNAD1 recombinant protein attenuates oxidative stress in neuronal cells through NADPH metabolism modulation"
**作者**: Chen R, et al.
**摘要**: 通过哺乳动物细胞表达体系获得高纯度OXNAD1重组蛋白，实验表明其通过调节NADPH代谢减轻神经元氧化损伤，为神经退行性疾病治疗提供新思路。
注：上述文献信息为示例性内容，实际研究中建议通过专业数据库核实具体文献。若需真实文献，可检索PubMed或Web of Science平台，使用关键词“OXNAD1 recombinant”或结合其基因别名(如C1orf105)进行精确查询。
OXNAD1 recombinant protein is derived from the OXNAD1 gene, which encodes a protein implicated in cellular metabolic processes, particularly those involving nicotinamide adenine dinucleotide (NAD+) biosynthesis or modulation. NAD+ is a critical coenzyme in redox reactions and energy metabolism, with emerging roles in aging, DNA repair, and stress response pathways. The OXNAD1 protein is hypothesized to interact with enzymes or regulators within NAD+ metabolic networks, though its precise mechanistic role remains under investigation. Dysregulation of NAD+ homeostasis is linked to age-related diseases, metabolic disorders, and neurodegenerative conditions, positioning OXNAD1 as a potential therapeutic target or biomarker.
Recombinant OXNAD1 protein is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell cultures, followed by purification via affinity chromatography. This engineered protein retains functional domains necessary for studying its biochemical interactions, enzymatic activity, or structural properties. Researchers utilize OXNAD1 recombinant protein to explore its involvement in NAD+-dependent pathways, including sirtuin activation, mitochondrial function, and calcium signaling. Its application extends to *in vitro* assays, antibody production, and drug screening platforms aimed at modulating NAD+ metabolism.
Current studies focus on clarifying OXNAD1's tissue-specific expression patterns, post-translational modifications, and cross-talk with other metabolic regulators like NMNATs or PARPs. Emerging evidence suggests its potential role in cancer cell survival and neuronal health, though functional data remain limited. As NAD+ biology gains traction in translational research, OXNAD1 recombinant protein serves as a vital tool for deciphering its contribution to metabolic resilience and disease pathogenesis, bridging gaps between basic biochemistry and therapeutic innovation.
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