| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLD5 |
| Uniprot No | Q8N7P1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 92-536aa |
| 氨基酸序列 | MGEDEDGLSEKNCQNKCRIALVENIPEGLNYSENAPFHLSLFQGWMNLLNMAKKSVDIVSSHWDLNHTHPSACQGQRLFEKLLQLTSQNIEIKLVSDVTADSKVLEALKLKGAEVTYMNMTAYNKGRLQSSFWIVDKQHVYIGSAGLDWQSLGQMKELGVIFYNCSCLVLDLQRIFALYSSLKFKSRVPQTWSKRLYGVYDNEKKLQLQLNETKSQAFVSNSPKLFCPKNRSFDIDAIYSVIDDAKQYVYIAVMDYLPISSTSTKRTYWPDLDAKIREALVLRSVRVRLLLSFWKETDPLTFNFISSLKAICTEIANCSLKVKFFDLERENACATKEQKNHTFPRLNRNKYMVTDGAAYIGNFDWVGNDFTQNAGTGLVINQADVRNNRSIIKQLKDVFERDWYSPYAKTLQPTKQPNCSSLFKLKPLSNKTATDDTGGKDPRNV |
| 预测分子量 | 66.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLD5重组蛋白的模拟参考文献示例(注：以下内容为虚构，实际文献请通过学术数据库核实)：
1. **文献名称**: "Expression and Functional Characterization of Recombinant PLD5 in Mammalian Cells"
**作者**: Smith A. et al.
**摘要**: 本研究利用哺乳动物表达系统成功表达了PLD5重组蛋白，并验证其磷脂酶活性。结果显示PLD5通过水解特定磷脂参与细胞膜信号传导，可能调控内质网应激反应。
2. **文献名称**: "Structural Insights into PLD5: Crystallographic Analysis of a Recombinant Human PLD5 Protein"
**作者**: Jones B. et al.
**摘要**: 通过X射线晶体学解析了人源PLD5重组蛋白的三维结构，揭示了其催化域的关键氨基酸残基，为设计靶向PLD5的小分子抑制剂提供了结构基础。
3. **文献名称**: "PLD5 Knockout and Recombinant Protein Rescue in a Neurodevelopmental Disorder Model"
**作者**: Chen L. et al.
**摘要**: 在小鼠模型中，PLD5基因缺失导致突触功能异常，而外源性重组PLD5蛋白的导入部分逆转了表型，提示其在神经发育中的潜在治疗价值。
4. **文献名称**: "Recombinant PLD5 Modulates Autophagy via mTOR Signaling Pathway"
**作者**: Kim S. et al.
**摘要**: 研究证明，体外纯化的PLD5重组蛋白通过抑制mTOR通路促进自噬，可能为癌症或神经退行性疾病提供新的干预靶点。
**注意**：以上文献信息为模拟生成，实际研究中请通过PubMed、Google Scholar等平台检索关键词(如“PLD5 recombinant protein”“phospholipase D5”)获取真实文献。
**Background of PLD5 Recombinant Protein**
Phospholipase D5 (PLD5) is a member of the phospholipase D (PLD) enzyme family, which plays critical roles in lipid metabolism, membrane trafficking, and cellular signaling. Unlike canonical PLD isoforms (e.g., PLD1 and PLD2) that hydrolyze phosphatidylcholine to generate phosphatidic acid (PA)—a key lipid secondary messenger—PLD5 exhibits distinct structural and functional characteristics. It contains two conserved HxKxxxxD motifs but lacks catalytic activity in classical PLD assays, suggesting divergent biological roles. PLD5 is expressed in various tissues, including the brain, and has been linked to neurodevelopment, autophagy, and cancer progression, though its precise mechanisms remain poorly understood.
Recombinant PLD5 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to enable functional and structural studies. By cloning the PLD5 gene into expression vectors, researchers produce purified, bioactive PLD5 protein, often tagged with affinity markers (e.g., His-tag) for efficient isolation. This recombinant tool has been pivotal in investigating PLD5’s interactions with lipids, proteins, or nucleic acids, as well as its potential regulatory effects on cellular pathways.
Recent studies highlight PLD5’s involvement in diseases. For example, altered PLD5 expression correlates with glioblastoma aggressiveness and neurodevelopmental disorders, implicating it as a biomarker or therapeutic target. However, challenges persist, including unresolved enzymatic activity, unclear substrate specificity, and contradictory roles in apoptosis versus survival.
Overall, PLD5 recombinant protein serves as a vital resource for decoding its enigmatic functions, bridging gaps between lipid biology and disease mechanisms, and guiding future therapeutic strategies.
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