| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RMDN3 |
| Uniprot No | Q96TC7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-470aa |
| 氨基酸序列 | MSRLGALGGARAGLGLLLGTAAGLGFLCLLYSQRWKRTQRHGRSQSLPNSLDYTQTSDPGRHVMLLRAVPGGAGDASVLPSLPREGQEKVLDRLDFVLTSLVALRREVEELRSSLRGLAGEIVGEVRCHMEENQRVARRRRFPFVRERSDSTGSSSVYFTASSGATFTDAESEGGYTTANAESDNERDSDKESEDGEDEVSCETVKMGRKDSLDLEEEAASGASSALEAGGSSGLEDVLPLLQQADELHRGDEQGKREGFQLLLNNKLVYGSRQDFLWRLARAYSDMCELTEEVSEKKSYALDGKEEAEAALEKGDESADCHLWYAVLCGQLAEHESIQRRIQSGFSFKEHVDKAIALQPENPMAHFLLGRWCYQVSHLSWLEKKTATALLESPLSATVEDALQSFLKAEELQPGFSKAGRVYISKCYRELGKNSEARWWMKLALELPDVTKEDLAIQKDLEELEVILRD |
| 预测分子量 | 56.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RMND3重组蛋白研究的示例性参考文献(注：RMND3为线粒体相关蛋白，可能与用户所指的"RMDN3"存在拼写差异，建议确认名称准确性)：
---
1. **文献名称**: *RMND3 regulates mitochondrial dynamics and induces apoptotic resistance in cancer cells*
**作者**: Li, X. et al. (2020)
**摘要**: 研究通过重组表达RMND3蛋白，发现其通过调控线粒体分裂-融合平衡，抑制线粒体膜电位下降，从而降低癌细胞凋亡敏感性，提示其在肿瘤治疗中的潜在靶点作用。
---
2. **文献名称**: *Recombinant RMND3 expression and its role in mitochondrial DNA maintenance*
**作者**: Garcia, S.M. & Zhang, Q. (2018)
**摘要**: 构建了重组RMND3蛋白表达系统，证实其与线粒体DNA聚合酶互作，参与mtDNA复制修复，突变体导致线粒体功能紊乱，为相关遗传病机制提供依据。
---
3. **文献名称**: *Purification and functional characterization of human RMND3 protein in yeast models*
**作者**: Watanabe, T. et al. (2019)
**摘要**: 利用酵母异源表达系统纯化人源RMND3重组蛋白，发现其可挽救酵母线粒体呼吸缺陷表型，证明其在氧化磷酸化复合体组装中的保守功能。
---
4. **文献名称**: *CRISPR/Cas9-mediated RMND3 knockout combined with recombinant protein rescue validates its role in neuronal development*
**作者**: Chen, L. et al. (2021)
**摘要**: 通过基因编辑结合重组RMND3回补实验，证实该蛋白通过调节线粒体动力学影响神经元突触形成，为神经退行性疾病研究提供新方向。
---
**注**: 以上为模拟文献示例。实际研究中建议通过PubMed/Google Scholar以**"RMND3 recombinant protein"**或**"RMDN3 gene function"**等关键词检索，并核对蛋白名称拼写及研究领域关联性。
RMDN3 (also known as RMND3 or Required for Meiotic Nuclear Division 3 homolog) is a conserved eukaryotic protein implicated in diverse cellular processes, particularly those involving microtubule dynamics and organelle organization. Originally identified in yeast for its role in meiotic spindle stability, the mammalian ortholog RMDN3 has gained attention for its broader regulatory functions. Structurally, it contains a conserved N-terminal domain critical for protein-protein interactions and a C-terminal region involved in microtubule binding. Studies suggest RMDN3 acts as an adaptor protein, bridging molecular motors (like dynein/dynactin complexes) with cellular cargoes during intracellular transport, mitochondrial network maintenance, and mitotic progression.
The recombinant RMDN3 protein, typically produced via bacterial (E. coli) or mammalian expression systems, enables detailed biochemical characterization. Its recombinant form preserves functional domains necessary for interaction with tubulin and motor proteins, making it valuable for in vitro assays studying cytoskeletal dynamics. Researchers utilize purified RMDN3 to investigate its role in neurodegenerative diseases (e.g., ALS, Parkinson's) where disrupted transport mechanisms contribute to pathology. Additionally, it serves as a tool to explore cancer cell division anomalies, given its mitotic regulatory functions. Recent work also links RMDN3 variants to rare genetic disorders involving neuromuscular dysfunction, driving interest in its therapeutic potential. Current challenges include resolving its precise molecular mechanisms and tissue-specific isoforms, areas where recombinant protein studies remain pivotal.
×
