| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ST8SIA4 |
| Uniprot No | Q92187 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-168aa |
| 氨基酸序列 | KTKEIARTEEHQETQLIGDGELSLSRSLVNSSDKIIRKAGSSIFQHNVEGWKINSSLVLEIRKNILRFLDAERDVSVVKSSFKPGDVIHYVLDRRRTLNISHDLHSLLPEVSPMKNRRFKTCAVVGNSGILLDSECGKEIDSHNFVIR |
| 预测分子量 | 32.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ST8SIA4重组蛋白的3篇参考文献的简化信息(注：内容基于公开研究背景模拟，非真实文献)：
1. **文献名称**: "ST8SIA4-mediated polysialylation regulates tumor metastasis via integrin signaling"
**作者**: Yamamoto K, et al.
**摘要**: 研究揭示了ST8SIA4重组蛋白通过催化细胞表面多糖唾液酸化修饰，调控整合素信号通路，促进肿瘤细胞迁移和转移的分子机制。
2. **文献名称**: "Recombinant ST8SIA4 protein enhances neurite outgrowth via GD3 ganglioside synthesis"
**作者**: Kawai H, et al.
**摘要**: 研究利用重组ST8SIA4蛋白在体外模型中验证其催化GD3神经节苷脂合成的功能，并证明其通过激活神经生长因子受体促进神经元突触生长。
3. **文献名称**: "Structural characterization of human ST8SIA4 recombinant enzyme for glycoengineering applications"
**作者**: Zhang L, et al.
**摘要**: 通过X射线晶体学解析了重组ST8SIA4蛋白的三维结构，阐明了其底物结合域的关键氨基酸残基，为糖工程改造提供了结构基础。
**提示**：实际文献需通过PubMed或Google Scholar检索关键词“ST8SIA4 recombinant protein”获取。建议结合研究场景筛选功能、疾病或结构相关论文。
ST8SIA4. also known as ST8 α-N-acetyl-neuraminide α-2.8-sialyltransferase 4. is a key enzyme in the biosynthesis of gangliosides, a class of glycosphingolipids enriched in the nervous system. This protein belongs to the sialyltransferase family, which catalyzes the transfer of sialic acid residues to glycan structures. Specifically, ST8SIA4 mediates the addition of α-2.8-linked polysialic acid (polySia) chains to neural cell adhesion molecules (NCAM) and other glycoproteins, playing critical roles in cell-cell interactions, neuronal plasticity, and developmental processes.
The recombinant ST8SIA4 protein is engineered for in vitro studies to investigate its enzymatic activity, substrate specificity, and regulatory mechanisms. Produced typically in mammalian or insect expression systems, it retains post-translational modifications essential for its function. Research has linked ST8SIA4 overexpression to tumor progression, particularly in neuroblastomas and gliomas, where polySia modifications enhance cell motility and invasiveness. Conversely, its dysregulation is implicated in neurodevelopmental disorders and psychiatric conditions.
Current applications of recombinant ST8SIA4 include structural studies to map catalytic domains, screening for inhibitors targeting cancer metastasis, and elucidating its role in neural regeneration. Challenges remain in resolving its full 3D structure and understanding context-dependent regulation. As ganglioside metabolism gains attention in immunotherapy and biomarker discovery, ST8SIA4 recombinant tools are increasingly valuable for both basic research and therapeutic development.
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