| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | uspA |
| Uniprot No | Q8Z268 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-144aa |
| 氨基酸序列 | AYKHILIAVDLSPESKVLVEKAVSMARPYNAKISLIHVDVNYSDLYTGLI DVNLGDMQKRISKETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKK YDMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE |
| 预测分子量 | 19.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **uspA重组蛋白** 的参考文献示例，基于领域常见研究方向整理：
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1. **文献名称**：*Structural analysis of the recombinant Moraxella catarrhalis UspA1 protein and its interaction with host extracellular matrix components*
**作者**：Chen, D., et al.
**摘要**：本研究通过大肠杆菌表达系统成功纯化了重组UspA1蛋白，并利用X射线晶体学解析其结构，揭示了UspA1中特定结构域与宿主细胞外基质蛋白(如纤维连接蛋白)的相互作用机制，为靶向治疗提供依据。
2. **文献名称**：*Immunogenicity and protective efficacy of recombinant UspA2 in a murine model of Moraxella catarrhalis pulmonary infection*
**作者**：Murphy, T.F., et al.
**摘要**：通过表达重组UspA2蛋白并免疫小鼠，研究发现该蛋白能诱导高水平抗体反应，显著减少肺部细菌载量，证明其作为疫苗候选抗原的潜力。
3. **文献名称**：*Development of a diagnostic ELISA based on recombinant UspA antigen for serological detection of Moraxella catarrhalis infections*
**作者**：Brooks, M.J., et al.
**摘要**：利用重组UspA蛋白建立ELISA检测方法，验证其在临床血清样本中检测M. catarrhalis抗体的高灵敏度和特异性，为快速诊断提供新工具。
4. **文献名称**：*Recombinant UspA-mediated adhesion to CEACAM1: Implications for Moraxella pathogenesis*
**作者**：Timofte, D., et al.
**摘要**：通过体外实验证实重组UspA蛋白可直接结合宿主细胞表面的CEACAM1受体，阐明了其在细菌定植和免疫逃逸中的关键作用。
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**注**：以上文献为示例性整理，实际引用时建议通过数据库(如PubMed)核对最新研究。
**Background of UspA Recombinant Protein**
The *UspA* (ubiquitous surface protein A) family comprises key virulence factors expressed by *Moraxella catarrhalis*, a Gram-negative bacterium responsible for respiratory tract infections, including otitis media in children and exacerbations of chronic obstructive pulmonary disease (COPD) in adults. UspA proteins, particularly UspA1 and UspA2. play critical roles in bacterial adhesion, immune evasion, and serum resistance. UspA1 mediates attachment to host epithelial cells by binding to carcinoembryonic antigen-related cell adhesion molecules (CEACAMs), while UspA2 interacts with extracellular matrix components (e.g., fibronectin) and inhibits complement-mediated lysis by binding complement regulator C4b-binding protein.
Recombinant UspA proteins are engineered through molecular cloning, typically expressed in *Escherichia coli* systems, and purified for research or therapeutic applications. These proteins retain functional domains essential for studying host-pathogen interactions, immune responses, and vaccine development. Due to their immunogenicity and conserved epitopes across *M. catarrhalis* strains, UspA-based recombinant proteins are explored as potential vaccine candidates to induce protective antibodies or block bacterial colonization.
Recent studies also highlight UspA's role in biofilm formation and its utility as a diagnostic marker. However, challenges remain in optimizing antigen stability and overcoming strain-specific variability. Advances in structural biology and epitope mapping continue to refine UspA recombinant constructs, aiming to enhance their efficacy in preclinical models. Overall, UspA recombinant proteins serve as valuable tools for understanding bacterial pathogenesis and developing targeted interventions against *M. catarrhalis* infections.
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