| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPAG16 |
| Uniprot No | Q8N0X2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-183aa |
| 氨基酸序列 | MAAQRGMPSSAVRVLEEALGMGLTAAGDARDTADAVAAEGAYYLEQVTITEASEDDYEYEEIPDDNFSIPEGEEDLAKAIQMAQEQATDTEILERKTVLPSKHAVPEVIEDFLCNFLIKMGMTRTLDCFQSEWYELIQKGVTELRTVGNVPDVYTQIMLLENENKNLKKDLKHYKQAAEYVIF |
| 预测分子量 | 47.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPAG16重组蛋白的3篇模拟参考文献示例(内容基于公开研究主题概括，实际文献需通过数据库验证)：
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1. **文献名称**：*SPAG16 regulates spermatogenesis through recombination protein expression in mice*
**作者**：Zhang Y, et al.
**摘要**：研究通过构建SPAG16重组蛋白，在小鼠模型中揭示其在精子形成中的关键作用，发现该蛋白缺失导致精子鞭毛结构异常和运动能力下降。
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2. **文献名称**：*Association of SPAG16 variants with male infertility via recombinant protein functional analysis*
**作者**：Li H, et al.
**摘要**：通过体外表达SPAG16重组蛋白，分析其与男性不育相关的基因突变，证实特定突变导致蛋白稳定性降低，影响精子发生过程。
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3. **文献名称**：*SPAG16 interacts with microtubule-associated proteins in a recombinant model system*
**作者**：Wang X, et al.
**摘要**：利用重组SPAG16蛋白进行互作实验，发现其与微管结合蛋白的相互作用对精子细胞骨架组装至关重要，为不育症机制提供新见解。
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**注**：以上为模拟概括，实际文献请通过PubMed、Google Scholar等平台以关键词“SPAG16 recombinant protein”或“SPAG16 spermatogenesis”检索，并核对作者及期刊信息。
**Background of SPAG16 Recombinant Protein**
SPAG16 (sperm-associated antigen 16) is a protein encoded by the *SPAG16* gene, primarily studied for its critical roles in spermatogenesis and ciliary function. It exists as two major splice variants: SPAG16L (long isoform) and SPAG16S (short isoform). SPAG16L, the larger isoform, localizes to the sperm flagellum and is essential for the structural integrity and motility of sperm. It interacts with other axonemal proteins, such as SPAG17. to regulate the assembly and stability of the sperm’s microtubule-based axoneme. SPAG16S, a nuclear isoform, is implicated in transcriptional regulation, though its precise mechanisms remain less defined.
The recombinant SPAG16 protein is engineered to mimic these isoforms for functional studies. Recombinant production typically involves expressing the protein in heterologous systems like *E. coli* or mammalian cells, enabling researchers to investigate its biochemical properties, protein interactions, and roles in cellular processes. Studies using SPAG16 recombinant proteins have highlighted its involvement in male infertility, as mutations or dysregulation of *SPAG16* correlate with impaired sperm motility and structural defects.
Beyond reproduction, SPAG16 has been linked to ciliopathies, disorders arising from dysfunctional cilia. Its axonemal role extends to motile cilia in respiratory and cerebral tissues, suggesting broader physiological impacts. Recombinant SPAG16 tools aid in exploring these pathways, offering insights into therapeutic strategies for infertility or cilia-related diseases.
Current research focuses on delineating SPAG16’s molecular interactions, post-translational modifications, and tissue-specific functions. The development of recombinant SPAG16 proteins continues to advance mechanistic studies and potential clinical applications, underscoring its significance in reproductive and cellular biology.
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