| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCDC36 |
| Uniprot No | Q8IYA8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 11-592aa |
| 氨基酸序列 | MLSIPSGSGNKKSSNWNNNQNDYSSLSDSQFLFGSQFCPENSETLSAPLDFGAHLRHSKQSQQNYLEGEPSIFTKYQTKPQLFGGDIKDGGLFPPPLSVGKSKGLLEQFEEKKKRAKDKCDSETLYNFVSNVRESILRLQTSVEKSEDHLSSRSQSILDSLETVAKTLQETIQAQNDLVFEAVQDKGNMEQAILEMKKRFEARQGEFIEMKSNLKHLEVLVAQQSQEFQQLCEQLGQLNVPSVLAELKRLISVPPVKDSASQTSPPLAQSLNLTRQEKYTSEKPVLWQAQALPAAWNPGMGSLQPGEFDVWGEGAKNDDLQEEAALPAFGSHERNRHVKDKVVQTNCKNWAVTKTGAKNHGSSVPGHKIPSDRDLVSQGASQLTSLEINFSTSIKNACQKYQAQSMFLCDPREHLVIKQKDGTVEMRGKDKKQQPRKAHRAHRGRLIASKQKQIPIQTCKFNSKYQSPQPAISVPQSPFLGQQEPRAQPLHLQCPRSPRKPVCPILGGTVMPNKTVRAVQGRLLQLSRCSSQDNWLLSSSSQGDHQMSWFSDLNLGCSETPLCKEAGKNLLYDLGFDSSDDD |
| 预测分子量 | 80.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCDC36重组蛋白的模拟参考文献示例(实际文献需通过学术数据库验证)：
1. **标题**: "Expression and Functional Analysis of Recombinant CCDC36 in Mammalian Cells"
**作者**: Zhang Y. et al.
**摘要**: 本研究在HEK293细胞中表达了重组CCDC36蛋白，并发现其通过调控纤毛形成参与细胞周期调控。重组蛋白的纯化及功能分析表明，CCDC36与微管结合蛋白相互作用，影响有丝分裂进程。
2. **标题**: "Structural Characterization of CCDC36 Using Recombinant Protein Expression in E. coli"
**作者**: Patel R. & Kumar S.
**摘要**: 作者在大肠杆菌中成功表达并纯化了CCDC36的重组蛋白，通过X射线晶体学解析其卷曲螺旋结构域，揭示了其潜在的蛋白质相互作用界面，为功能研究提供结构基础。
3. **标题**: "CCDC36 Recombinant Protein Interacts with Ciliary Transport Complexes: Implications for Genetic Disorders"
**作者**: Lee H. et al.
**摘要**: 该研究利用昆虫细胞表达系统制备重组CCDC36蛋白，发现其与纤毛运输复合物IFT20直接结合，提示CCDC36缺陷可能导致纤毛相关遗传疾病，如多囊肾。
4. **标题**: "Functional Screening of CCDC36 Variants via Recombinant Protein Assays"
**作者**: Gomez F. et al.
**摘要**: 通过重组表达野生型和突变型CCDC36蛋白，结合体外生化实验，筛选出与癌症相关的功能丧失突变，证实CCDC36在DNA损伤修复中的潜在作用。
**注意**：以上内容为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索确认。若研究较新或较冷门，建议扩展关键词(如“CCDC36 gene cloning”“coiled-coil domain protein 36”)或联系领域专家获取资源。
**Background of CCDC36 Recombinant Protein**
CCDC36 (Coiled-Coil Domain-Containing Protein 36) is a less characterized member of the coiled-coil domain-containing protein family, which plays diverse roles in cellular processes such as cytoskeletal organization, intracellular transport, and signal transduction. The CCDC36 gene encodes a protein predicted to contain multiple α-helical coiled-coil motifs, structural features often involved in protein-protein interactions and scaffolding. While its precise biological function remains under investigation, CCDC36 is hypothesized to participate in maintaining cellular architecture or regulating molecular complexes due to its structural domains.
Recombinant CCDC36 protein is artificially produced using expression systems like *E. coli* or mammalian cell lines, enabling large-scale purification for functional and structural studies. Its recombinant form typically includes affinity tags (e.g., His-tag) to facilitate isolation and detection. Researchers employ this tool to explore CCDC36's interactions with other biomolecules, its subcellular localization, and potential roles in pathways linked to human diseases.
Emerging studies suggest associations between CCDC family proteins and pathologies, including ciliopathies and cancer, though direct evidence for CCDC36's involvement is limited. Investigating recombinant CCDC36 could clarify its contribution to cellular homeostasis or disease mechanisms, aiding drug discovery or diagnostic development. Current research focuses on mapping its interactome, resolving 3D structures, and validating its physiological relevance using knockout models or biochemical assays.
In summary, CCDC36 recombinant protein serves as a critical reagent to unravel the functional significance of this understudied protein, bridging gaps in understanding coiled-coil domain proteins' roles in health and disease.
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