| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OFCC1 |
| Uniprot No | Q8IZS5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 3-231aa |
| 氨基酸序列 | REKFQQKALKQTKQKKSKSAEFLMVKEDREATEGTGNPAFNMSSPDLSACQTAEKKVIRHDMPDRTLAAHQQKFRLPASAEPKGNEYGRNYFDPLMDEEINPRQCATEVSREDDDRIFYNRLTKLFDESRQGEPQDESGREETLNSEAPGSSNKSHEIHKEASEATTAHLEEFQRSQKTIILLGSSPLEQEIRSTSLHCMEDEMSHPWILLLKVTAVIRSRRYYREQRF |
| 预测分子量 | 42.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OFCC1重组蛋白的模拟参考文献示例(注：以下内容为假设性示例，实际文献需通过学术数据库验证)：
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1. **标题**：*Cloning, Expression, and Functional Analysis of Recombinant OFCC1 in Osteoclastogenesis*
**作者**：Zhang L, et al. (2020)
**摘要**：本研究成功克隆人源OFCC1基因，在大肠杆菌中实现重组表达并纯化。功能实验表明，重组OFCC1通过调控细胞骨架蛋白相互作用，显著影响破骨细胞融合过程。
2. **标题**：*Structural Characterization of OFCC1 Recombinant Protein by Cryo-EM*
**作者**：Smith RJ, et al. (2021)
**摘要**：利用冷冻电镜技术解析了重组OFCC1蛋白的三维结构，揭示了其N端结构域与细胞黏附相关的关键功能位点，为靶向药物设计提供依据。
3. **标题**：*OFCC1 Recombinant Protein Inhibits Tumor Metastasis via Modulating Focal Adhesion Kinase Signaling*
**作者**：Li X, et al. (2019)
**摘要**：通过哺乳动物表达系统制备重组OFCC1.发现其可抑制乳腺癌细胞迁移和侵袭，机制研究与黏着斑激酶(FAK)信号通路调控相关。
4. **标题**：*Optimization of OFCC1 Recombinant Production in Insect Cells for Functional Studies*
**作者**：Wang Y, et al. (2018)
**摘要**：在昆虫细胞中优化OFCC1重组蛋白表达条件，获得高纯度活性蛋白，并验证其在体外模型中对细胞极性的调控作用。
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建议通过 **PubMed/Google Scholar** 检索真实文献，关键词可尝试：
`OFCC1 recombinant protein`、`OFCC1 expression and purification`、`OFCC1 functional study`。
**Background of OFCC1 Recombinant Protein**
OFCC1 (Outer Dense Fiber Coiled-Coil Protein 1) is a structural protein predominantly localized in the outer dense fibers (ODFs) of sperm flagella. These fibers are critical for maintaining the mechanical stability and elastic integrity of sperm tails, enabling proper motility and function during fertilization. Mutations or dysregulation of OFCC1 have been linked to male infertility due to impaired sperm motility or structural abnormalities.
Recombinant OFCC1 protein is produced using biotechnological methods, often by expressing the OFCC1 gene in bacterial or mammalian cell systems. The gene is cloned into expression vectors, followed by purification via affinity chromatography (e.g., His-tag systems) and validation through techniques like SDS-PAGE and Western blot. This engineered protein retains the native structure and functional domains of endogenous OFCC1. allowing researchers to study its biological roles in vitro or in cellular models.
Research applications of OFCC1 recombinant protein include investigating sperm tail assembly mechanisms, protein-protein interactions within the ODF complex, and pathways regulating spermatogenesis. It also serves as a tool for developing diagnostic assays for male infertility or screening potential therapeutic agents targeting sperm-related disorders. Additionally, studies explore OFCC1’s potential roles beyond reproduction, such as its expression in certain cancers or ciliated cells, though these areas remain less characterized.
Overall, OFCC1 recombinant protein provides a valuable resource for advancing reproductive biology and understanding structural protein networks critical for cellular motility and function.
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