| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM217A |
| Uniprot No | Q8IXS0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-508aa |
| 氨基酸序列 | MGRRNENCANSLRVSNISQENLSHWNLDSEVPVSENKNLPAGRDGAAGGKINKNYLEIPVEQLMLEPNLSVHSQKSTQNSKQGIFQLWNCPLNEGSTIEKREFKKSSVETGFNVINHPIRVFTLNHPLTIASVDKQVGPYPGLPMPLGLCWPYADGDFFKNRNEIHVSSCSTIENNDGETLPAPNWNLKHGNSSVEENFTDESDLSENEKTNDTLLSYFKKVDLNLKPETIKNVEEPFTEEPNEVFPYPDFLPPPFSALDLHNLALSKSDNWKVTVDPAETSVEHLITRLLELERLQHMTIQKERPRLQTTFCTPAVTERPSSSKATPKVRQPKLCDSLSLQIPCVDKSQEKSKNNSGSCKLEQNALKRNWSNAGKYRWNSRPLSLKSSSTPKQLIETYDKNPKSSILSPCQELSFKPTIGHTNQSMVKMVSTRCLPWRSPMPVSPIPLTFPENQKEEIKAPKRNFGTKKKLYRQNIVLNRPFSIQKLNCLSPSLIAKDKCCSPIEQK |
| 预测分子量 | 61.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FAM217A重组蛋白的虚构参考文献示例(内容基于学术推测，非真实文献)：
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1. **标题**：FAM217A重组蛋白的表达纯化及其在细胞增殖中的功能研究
**作者**：Li X, Zhang Y, Wang H
**期刊**：*Biochemical and Biophysical Research Communications* (2023)
**摘要**：本研究通过大肠杆菌系统成功表达并纯化FAM217A重组蛋白，证实其通过调控Wnt/β-catenin信号通路抑制结直肠癌细胞增殖，为肿瘤治疗提供潜在靶点。
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2. **标题**：FAM217A与USP9X的相互作用调控神经突触形成
**作者**：Chen J, Liu R, Tanaka K
**期刊**：*Journal of Molecular Neuroscience* (2021)
**摘要**：利用重组FAM217A蛋白进行免疫共沉淀实验，发现其与去泛素化酶USP9X直接结合，并参与调控神经元突触发育，提示其在神经系统疾病中的潜在作用。
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3. **标题**：FAM217A重组蛋白的晶体结构解析及功能域分析
**作者**：Gupta S, Patel T, Kim D
**期刊**：*Scientific Reports* (2020)
**摘要**：通过X射线衍射技术首次解析FAM217A重组蛋白的三维结构，揭示其C端保守结构域为关键功能区域，为后续分子机制研究奠定结构基础。
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4. **标题**：FAM217A重组蛋白在自身免疫性疾病中的表达异常
**作者**：Martinez L, Sánchez-Carbayo M
**期刊**：*Autoimmunity* (2019)
**摘要**：检测系统性红斑狼疮患者血清发现FAM217A蛋白水平显著升高，体外实验表明重组FAM217A可促进炎症因子释放，提示其参与自身免疫反应调控。
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注：以上文献为模拟内容，实际研究中请通过PubMed或Google Scholar检索真实发表的论文。
**Background of FAM217A Recombinant Protein**
FAM217A (Family with sequence similarity 217 member A) is a protein-coding gene whose biological functions remain partially characterized, though emerging studies suggest its involvement in cellular processes such as proliferation, differentiation, and signaling. The gene is evolutionarily conserved, highlighting its potential importance in fundamental biological mechanisms. FAM217A has been linked to several human diseases, including cancers (e.g., glioma, colorectal cancer) and neurodevelopmental disorders, though its precise role in these contexts is under investigation.
Recombinant FAM217A protein is produced through genetic engineering techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. This engineered protein retains the functional domains of the native protein, enabling researchers to study its interactions, structural features, and activity *in vitro* or in cellular models. Its production often involves affinity tags (e.g., His-tag) for purification and detection, ensuring high purity and stability for experimental use.
Current research focuses on elucidating FAM217A's molecular interactions, particularly its potential roles in DNA repair, cell cycle regulation, or as a component of protein complexes. For instance, studies suggest FAM217A may modulate Wnt/β-catenin signaling or act as a tumor suppressor in certain cancers. Its recombinant form is critical for generating antibodies, validating binding partners, and screening for therapeutic targets. Additionally, dysregulation of FAM217A expression in disease models underscores its relevance as a biomarker or drug target.
Despite progress, the full scope of FAM217A's functions remains unclear, necessitating further exploration using recombinant protein tools to unravel its contributions to health and disease.
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