| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Dock8 |
| Uniprot No | Q8NF50 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 560-729aa |
| 氨基酸序列 | RNLLYVYPQRLNFVNKLASARNITIKIQFMCGEDASNAMPVIFGKSSGPE FLQEVYTAVTYHNKSPDFYEEVKIKLPAKLTVNHHLLFTFYHISCQQKQG ASVETLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSMHSAEKVPLQN PPIKWAEGHKGVFNIEVQAV |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于DOCK8重组蛋白的参考文献摘要简述：
1. **文献名称**：*DOCK8 regulates T cell migration and phagosomal escape in dendritic cells*
**作者**：Zhang Q. et al.
**摘要**：研究利用重组DOCK8蛋白揭示其在T细胞迁移中的关键作用，证明DOCK8缺陷导致树突状细胞吞噬体逃逸功能受损，进而引发免疫缺陷。
2. **文献名称**：*Structural basis of DOCK8-mediated signaling in antiviral immunity*
**作者**：Engelhardt K.R., et al.
**摘要**：通过重组DOCK8蛋白的晶体结构分析，阐明其通过CDC42信号通路调控淋巴细胞极化，解释DOCK8缺失患者易患病毒感染的分子机制。
3. **文献名称**：*DOCK8 recombination repair in hyper-IgE syndrome*
**作者**：Janssen E., et al.
**摘要**：利用重组DOCK8蛋白修复模型，发现其能够恢复STAT3信号通路缺陷，改善高IgE综合征患者的Th17细胞分化异常问题。
注：上述文献为示例性内容，实际文献需通过PubMed或Sci-Hub等学术平台检索确认。
**Background of DOCK8 Recombinant Protein**
DOCK8 (Dedicator of Cytokinesis 8) is a member of the DOCK protein family, which functions as atypical guanine nucleotide exchange factors (GEFs) that activate small GTPases like Rac and Cdc42. These GTPases regulate cytoskeletal dynamics, cell migration, and immune responses. DOCK8 is particularly critical in immune cell signaling, including lymphocyte trafficking, dendritic cell maturation, and T-cell function. Mutations in the *DOCK8* gene are linked to a rare autosomal recessive form of hyper-IgE syndrome (HIES), characterized by severe immunodeficiency, recurrent infections, and allergic manifestations.
Recombinant DOCK8 protein is engineered in vitro to study its biochemical and functional properties. It typically includes conserved domains such as the DHR-2 (Dock Homology Region 2), responsible for GEF activity, and the PH (Pleckstrin Homology) domain, which mediates membrane interactions. Researchers produce this protein using expression systems (e.g., mammalian, insect, or bacterial cells) followed by purification techniques like affinity chromatography.
Studies employing DOCK8 recombinant protein aim to dissect its role in immune regulation, GTPase activation mechanisms, and disease pathology. It serves as a tool for screening therapeutic compounds targeting DOCK8-related disorders or enhancing immune function. Additionally, recombinant DOCK8 aids in structural studies to resolve its 3D architecture, guiding the design of gene therapies or biologics for patients with DOCK8 deficiency.
Overall, DOCK8 recombinant protein is pivotal in advancing our understanding of immune dysregulation and developing precision treatments for rare genetic immunodeficiencies.
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