| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Rbfox3 |
| Uniprot No | A6NFN3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-312aa |
| 氨基酸序列 | MAQPYPPAQY PPPPQNGIPA EYAPPPPHPT QDYSGQTPVP TEHGMTLYTP AQTHPEQPGS EASTQPIAGT QTVPQTDEAA QTDSQPLHPS DPTEKQQPKR LHVSNIPFRF RDPDLRQMFG QFGKILDVEI IFNERGSKGF GFVTFETSSD ADRAREKLNG TIVEGRKIEV NNATARVMTN KKTGNPYTNG WKLNPVVGAV YGPEFYAVTG FPYPTTGTAV AYRGAHLRGR GRAVYNTFRA APPPPPIPTY GAVVYQDGFY GAEIYGGYAA YRYAQPAAAA AAYSDSYGRV YAAADPYHHT IGPAATYSIG TM |
| 预测分子量 | 33,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Rbfox3重组蛋白的3篇参考文献示例(文献信息为虚拟概括，仅作参考)：
---
1. **"Rbfox3 regulates alternative splicing in neuronal differentiation"**
*作者：Kim et al. (2015)*
**摘要**：研究利用重组Rbfox3蛋白，揭示其在神经元分化过程中通过调控靶基因(如突触相关蛋白)的pre-mRNA选择性剪接，促进神经细胞成熟的作用机制。
2. **"Functional characterization of recombinant Rbfox3 in RNA binding assays"**
*作者：Gehman et al. (2012)*
**摘要**：通过体外表达纯化Rbfox3重组蛋白，结合电泳迁移实验(EMSA)和CLIP-seq技术，证明其特异性结合RNA的UGCAUG基序，并调控神经特异性剪接事件。
3. **"Rbfox3 deficiency and rescue by recombinant protein in neurodegeneration models"**
*作者：Huang et al. (2018)*
**摘要**：在阿尔茨海默病细胞模型中，外源性添加重组Rbfox3蛋白可部分恢复tau蛋白异常剪接，缓解神经元凋亡，提示其潜在治疗价值。
---
**注**：以上文献为示例性概括，实际研究中需根据具体实验方向检索真实文献(可参考PubMed或SciHub平台)。
Rbfox3. also known as neuronal nuclei antigen (NeuN), is a member of the RNA-binding Fox (Rbfox) protein family, characterized by a conserved RNA recognition motif (RRM) that enables sequence-specific interactions with target RNAs. This protein is predominantly expressed in post-mitotic neurons, where it plays critical roles in regulating alternative splicing, mRNA stability, and translation. As a key RNA-binding protein, Rbfox3 contributes to neurodevelopment, synaptic plasticity, and neuronal function by modulating the expression of genes involved in axon guidance, ion channel activity, and synaptic transmission.
The recombinant Rbfox3 protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or mammalian cell systems to ensure proper folding and post-translational modifications. Purification methods often involve affinity tags (e.g., His-tag) for high-yield isolation. Recombinant Rbfox3 serves as a vital tool for studying RNA-protein interactions, spliceosome mechanisms, and neuronal differentiation in vitro. Its application extends to disease research, particularly neurodegenerative disorders (e.g., Alzheimer’s, epilepsy) and neurodevelopmental conditions linked to Rbfox3 dysregulation or mutations.
Notably, Rbfox3/NeuN is widely used as a biomarker in immunohistochemistry to identify mature neurons, though its recombinant form enables standardized quantification in experimental models. Recent studies highlight its role in stress granule formation and responses to neuronal injury, expanding its relevance in cellular stress pathways. By leveraging recombinant Rbfox3. researchers aim to unravel its therapeutic potential in restoring RNA processing defects underlying neurological diseases.
×
