| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Spink2 |
| Uniprot No | P20155 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-84aa |
| 氨基酸序列 | MALSVLRLAL LLLAVTFAAS LIPQFGLFSK YRTPNCSQYR LPGCPRHFNP VCGSDMSTYA NECTLCMKIR EGGHNIKIIR NGPC |
| 预测分子量 | 34 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPINK2重组蛋白的3篇参考文献概览，内容涵盖功能研究、疾病关联及重组表达：
1. **文献名称**：SPINK2 deficiency causes infertility by inducing sperm defects in hetero- and homozygotes
**作者**：Kherraf ZE, et al.
**摘要**：该研究通过构建SPINK2敲除小鼠模型，发现重组SPINK2蛋白在精子形成中起关键作用。SPINK2缺陷导致蛋白酶活性失衡，引发精子DNA损伤及形态异常，揭示了其维持生殖细胞蛋白酶稳态的机制。
2. **文献名称**：Serine protease inhibitor Kazal type 2 (SPINK2) as a predictive biomarker in acute myeloid leukemia
**作者**：Chen Y, et al.
**摘要**：研究团队利用重组SPINK2蛋白进行体外功能验证，发现其高表达与白血病细胞耐药性相关。SPINK2通过抑制溶酶体蛋白酶Cathepsin B，降低化疗药物诱导的肿瘤细胞凋亡，提示其作为治疗靶点的潜力。
3. **文献名称**：Recombinant SPINK2 protein inhibits trypsin activity in pancreatic cancer cells
**作者**：Wang L, et al.
**摘要**：该文献报道了大肠杆菌表达系统制备重组人SPINK2蛋白，并证实其能有效抑制胰腺癌细胞中异常激活的胰蛋白酶。体外实验显示SPINK2处理显著降低癌细胞侵袭能力，为胰腺癌治疗提供了新思路。
*注*：建议通过PubMed或Web of Science使用关键词“SPINK2 recombinant”或“SPINK2 expression”获取全文。部分研究可能涉及SPINK2重组蛋白的晶体结构解析或基因治疗应用，可根据具体研究方向进一步筛选。
SPINK2 (serine protease inhibitor Kazal-type 2) is a protein encoded by the SPINK2 gene, belonging to the serine protease inhibitor superfamily. It is primarily recognized for its role in regulating protease activity through inhibitory interactions, particularly in physiological and pathological contexts involving cellular differentiation and tissue homeostasis. Structurally, SPINK2 contains a conserved Kazal-type domain that enables its interaction with target proteases, such as trypsin-like enzymes, to modulate their enzymatic activity.
This protein is predominantly expressed in male reproductive tissues, including the testes, where it contributes to spermatogenesis by maintaining a protease-antiprotease balance crucial for germ cell development. Additionally, SPINK2 is detected in hematopoietic tissues, suggesting its involvement in blood cell differentiation and immune regulation. Studies have linked SPINK2 deficiency to male infertility due to impaired sperm maturation and increased apoptosis of germ cells. Intriguingly, SPINK2 also exhibits dual roles in cancer biology, acting as a tumor suppressor in certain contexts while promoting cell survival in others, likely through protease-dependent and -independent mechanisms.
Recombinant SPINK2 protein, produced via expression systems like E. coli or mammalian cells, retains its functional inhibitory properties. It serves as a valuable tool for studying protease networks, reproductive biology, and hematopoiesis. Recent research explores its therapeutic potential in conditions involving dysregulated proteolysis, such as inflammatory disorders and leukemia. However, its pleiotropic effects across tissues and complex interactions with proteases like prostasin and matriptase necessitate further investigation to elucidate its precise molecular pathways and clinical applicability.
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