| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MON1A |
| Uniprot No | Q86VX9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 205-555aa |
| 氨基酸序列 | DGYKVVFVRRSPLVLVAVARTRQSAQELAQELLYIYYQILSLLTGAQLSHIFQQKQNYDLRRLLSGSERITDNLLQLMARDPSFLMGAARCLPLAAAVRDTVSASLQQARARSLVFSILLARNQLVALVRRKDQFLHPIDLHLLFNLISSSSSFREGEAWTPVCLPKFNAAGFFHAHISYLEPDTDLCLLLVSTDREDFFAVSDCRRRFQERLRKRGAHLALREALRTPYYSVAQVGIPDLRHFLYKSKSSGLFTSPEIEAPYTSEEEQERLLGLYQYLHSRAHNASRPLKTIYYTGPNENLLAWVTGAFELYMCYSPLGTKASAVSAIHKLMRWIRKEEDRLFILTPLTY |
| 预测分子量 | 56.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MON1A重组蛋白的3篇代表性文献(注：以下内容为模拟虚构，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: "Functional characterization of MON1A-CCZ1 complex in autophagosome-lysosome fusion"
**作者**: Smith J, et al.
**摘要**: 本研究通过表达并纯化人源MON1A重组蛋白，发现其与CCZ1形成复合体，调控自噬过程中溶酶体膜融合的关键步骤，揭示了其在细胞自噬中的分子机制。
2. **文献名称**: "Recombinant MON1A protein production in E. coli and its GTPase activity analysis"
**作者**: Lee S, Kim D.
**摘要**: 报道了利用大肠杆菌系统高效表达MON1A重组蛋白的优化方法，并通过体外实验证明MON1A具有GTP酶活性，可能参与细胞内囊泡运输的信号传导。
3. **文献名称**: "MON1A overexpression correlates with poor prognosis in colorectal cancer"
**作者**: Zhang Y, et al.
**摘要**: 通过重组MON1A蛋白制备抗体，分析其在结直肠癌组织中的表达水平，发现MON1A高表达与患者生存率降低显著相关，提示其作为潜在肿瘤标志物的可能性。
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如需具体文献，建议通过PubMed、Web of Science等平台以“MON1A recombinant protein”或“MON1A autophagy”为关键词检索。
MON1A (Membrane protein associated with chromosome 1A) is a member of the MON1/SAND family of proteins, which play conserved roles in membrane trafficking and autophagy. This gene encodes a regulatory component of the HOPS (homotypic fusion and vacuole protein sorting) complex, essential for endosomal-lysosomal fusion and lysosomal maturation. MON1A forms a heterodimer with CCZ1. which activates Rab7 GTPase to facilitate vesicle tethering and cargo transport. Dysregulation of MON1A is implicated in cellular processes linked to cancer progression, neurodegenerative diseases, and immune responses.
Recombinant MON1A protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables functional studies of its interaction networks and structural characterization. Its application spans in vitro assays investigating endosomal trafficking, autophagy flux measurements, and drug screening platforms targeting lysosome-related disorders. Recent studies highlight MON1A's role in tumor metastasis through Rab7-mediated integrin recycling in hepatocellular carcinoma, while its deficiency correlates with disrupted lysosomal acidification in neurodegenerative models. As a research tool, purified MON1A protein also aids in antibody development for diagnostic biomarkers. Current challenges include elucidating post-translational modifications and tissue-specific isoform functions, emphasizing the need for high-purity recombinant variants to advance therapeutic targeting strategies.
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