| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Septin9 |
| Uniprot No | Q80UG5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-583aa |
| 氨基酸序列 | MKKSYSGVTRTSSGRLRRLADPTGPALKRSFEVEEIEPPNSTPPRRVQTPLLRATVASSSQKFQDLGVKNSEPAARLVDSLSQRSPKPSLRRVELAGAKAPEPMSRRTEISIDISSKQVESTASAAGPSRFGLKRAEVLGHKTPEPVPRRTEITIVKPQESVLRRVETPASKIPEGSAVPATDAAPKRVEIQVPKPAEAPNCPLPSQTLENSEAPMSQLQSRLEPRPSVAEVPYRNQEDSEVTPSCVGDMADNPRDAMLKQAPASRNEKAPMEFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVYFKQRITADLLSNGIDVYPQKEFDEDAEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSNIHFEAYRVKRLNEGNSAMANGIEKEPEAQEM |
| 预测分子量 | 69.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Septin9重组蛋白的3篇代表性文献的简要列举(注：文献为模拟示例，非真实存在)：
1. **《Expression and purification of recombinant human Septin9 in Escherichia coli for structural studies》**
*作者：Zhang L, et al.*
**摘要**：该研究报道了在大肠杆菌系统中高效表达人源Septin9重组蛋白的优化策略，采用亲和层析和凝胶过滤技术纯化获得高纯度蛋白，并通过圆二色谱验证其二级结构完整性，为后续结构功能研究奠定基础。
2. **《Functional characterization of Septin9 in cytoskeletal reorganization using reconstituted recombinant complexes》**
*作者：Mendoza M, et al.*
**摘要**：通过共表达Septin9与其他septin家族成员(如SEPT2/6/7)，在体外重构了septin异源复合体，并利用荧光显微技术揭示Septin9在微管结合及细胞骨架动态调控中的关键作用，提示其与肿瘤细胞迁移的相关性。
3. **《Development of a Septin9 recombinant protein-based ELISA for early detection of colorectal cancer》**
*作者：Wang Y, et al.*
**摘要**：研究利用重组Septin9蛋白作为抗原，开发了一种高灵敏度ELISA检测方法，可特异性识别患者血清中抗Septin9自身抗体，在临床试验中显示出优于传统甲基化DNA检测的诊断效能，为无创癌症筛查提供新策略。
*注：以上文献信息为基于领域知识的合理推演，实际引用时请以真实数据库(如PubMed)检索结果为准。*
**Background of Septin9 Recombinant Protein**
Septin9. a member of the septin family of GTP-binding proteins, plays critical roles in cytoskeletal organization, cell division, membrane dynamics, and intracellular transport. This family is evolutionarily conserved and implicated in diverse cellular processes, including cytokinesis, apoptosis, and vesicle trafficking. Septin9 (SEPT9) is encoded by the *SEPTIN9* gene, located on human chromosome 17q25.3. and undergoes alternative splicing to produce multiple isoforms with distinct functional and regulatory properties. Dysregulation of SEPT9 expression or mutations in its gene have been linked to cancers (e.g., colorectal, ovarian), neurodegenerative disorders, and cardiovascular diseases, highlighting its biomedical significance.
Recombinant Septin9 protein is artificially produced using expression systems (e.g., *E. coli*, mammalian cells) to obtain purified, functional protein for research and diagnostic applications. Its production involves cloning the *SEPTIN9* gene into expression vectors, followed by protein purification via affinity chromatography. Recombinant Septin9 retains biochemical properties of native protein, enabling studies on its structure, interactions (e.g., with actin, microtubules, or other septins), and role in disease mechanisms.
In cancer research, Septin9 is a biomarker; hypermethylation of its promoter is a diagnostic marker for colorectal cancer. Recombinant Septin9 aids in developing assays to detect methylation or protein expression levels. It also serves as a tool for screening therapeutic agents targeting septin-mediated pathways. Despite progress, challenges remain in understanding isoform-specific functions and regulatory networks. Ongoing studies aim to elucidate its role in cellular homeostasis and disease, driving innovations in diagnostics and targeted therapies.
×
