| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DDX53 |
| Uniprot No | Q86TM3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-631aa |
| 氨基酸序列 | MSHWAPEWKRAEANPRDLGASWDVRGSRGSGWSGPFGHQGPRAAGSREPPLCFKIKNNMVGVVIGYSGSKIKDLQHSTNTKIQIINGESEAKVRIFGNREMKAKAKAAIETLIRKQESYNSESSVDNAASQTPIGRNLGRNDIVGEAEPLSNWDRIRAAVVECEKRKWADLPPVKKNFYIESKATSCMSEMQVINWRKENFNITCDDLKSGEKRLIPKPTCRFKDAFQQYPDLLKSIIRVGIVKPTPIQSQAWPIILQGIDLIVVAQTGTGKTLSYLMPGFIHLDSQPISREQRNGPGMLVLTPTRELALHVEAECSKYSYKGLKSICIYGGRNRNGQIEDISKGVDIIIATPGRLNDLQMNNSVNLRSITYLVIDEADKMLDMEFEPQIRKILLDVRPDRQTVMTSATWPDTVRQLALSYLKDPMIVYVGNLNLVAVNTVKQNIIVTTEKEKRALTQEFVENMSPNDKVIMFVSQKHIADDLSSDFNIQGISAESLHGNSEQSDQERAVEDFKSGNIKILITTDIVSRGLDLNDVTHVYNYDFPRNIDVYVHRVGYIGRTGKTGTSVTLITQRDSKMAGELIKILDRANQSVPEDLVVMAEQYKLNQQKRHRETRSRKPGQRRKEFYFLS |
| 预测分子量 | 76.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于DDX53重组蛋白的模拟参考文献示例(注：内容为虚构，仅供格式参考)：
---
1. **文献名称**: *Expression and Functional Analysis of Recombinant DDX53 in Cancer Cells*
**作者**: Smith A.B., et al.
**摘要**: 本研究利用大肠杆菌表达系统成功纯化DDX53重组蛋白，并验证其RNA解旋酶活性。实验发现DDX53在结肠癌细胞中异常高表达，并通过调控特定mRNA稳定性促进肿瘤增殖，提示其作为癌症治疗靶点的潜力。
2. **文献名称**: *Structural Characterization of Human DDX53 Helicase Domain*
**作者**: Chen L., Wang X., & Tanaka K.
**摘要**: 通过X射线晶体学解析DDX53蛋白的ATP结合结构域三维结构，揭示其与ATP类似物的相互作用位点。重组蛋白功能实验表明，关键氨基酸突变会显著降低其解旋酶活性，为开发小分子抑制剂提供结构基础。
3. **文献名称**: *DDX53 Recombinant Protein as a Biomarker in Hepatocellular Carcinoma*
**作者**: Gupta R., et al.
**摘要**: 采用哺乳动物细胞表达系统制备DDX53重组蛋白，开发高灵敏度ELISA检测方法。临床样本分析显示，肝癌患者血清中DDX53水平显著升高，提示其可作为早期诊断的生物标志物。
---
注：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science等平台检索真实文献。
DDX53 (DEAD-box helicase 53), also known as CAGE1 (cancer-associated gene 1), is a member of the DEAD-box RNA helicase family characterized by conserved Asp-Glu-Ala-Asp (DEAD) motifs. These proteins play roles in RNA metabolism, including unwinding RNA secondary structures, ribosome biogenesis, and mRNA splicing. DDX53 is distinctively associated with cancer biology, where its abnormal expression has been linked to tumorigenesis and therapeutic resistance. It is frequently overexpressed in various malignancies, including hepatocellular carcinoma, colorectal cancer, and melanoma, and is implicated in promoting cell proliferation, metastasis, and immune evasion.
The recombinant DDX53 protein is engineered for research applications to study its molecular mechanisms and interactions. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), the recombinant form retains functional domains, such as the ATP-binding and helicase domains, enabling in vitro analysis of its enzymatic activity, RNA-binding properties, and role in signaling pathways. Its structural features, including the conserved DEAD-box motif, make it a target for investigating helicase-driven processes in cancer cells.
Studies using recombinant DDX53 have highlighted its potential as a biomarker or therapeutic target. For instance, it interacts with tumor-associated antigens, suggesting utility in cancer immunotherapy. Additionally, recombinant DDX53 aids in screening inhibitors that block its helicase activity or disrupt protein-RNA interactions, offering avenues for drug development. Despite progress, its precise biological roles and regulatory networks remain under exploration, necessitating further structural and functional studies to clarify its dual roles in RNA processing and cancer progression. This protein thus represents a bridge between RNA biology and oncogenic mechanisms, with translational implications in diagnostics and therapy.
×
