| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT77 |
| Uniprot No | Q7Z794 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-578aa |
| 氨基酸序列 | MSHQFSSQSAFSSMSRRVYSTSSSAGSGGGSPAVGSVCYARGRCGGGGYGIHGRGFGSRSLYNLGGSRSISINLMGRSTSGFCQGGGVGGFGGGRGFGVGSTGAGGFGGGGFGGAGFGTSNFGLGGFGPYCPPGGIQEVTINQSLLEPLHLEVDPEIQRIKTQEREQIMVLNNKFASFIDKVRFLEQQNQVLQTKWELLQQVNTSTGTNNLEPLLENYIGDLRRQVDLLSAEQMRQNAEVRSMQDVVEDYKSKYEDEINKRTGSENDFVVLKKDVDAAYVSKVDLESRVDTLTGEVNFLKYLFLTELSQVQTHISDTNVILSMDNNRSLDLDSIIDAVRTQYELIAQRSKDEAEALYQTKYQELQITAGRHGDDLKNSKMEIAELNRTVQRLQAEISNVKKQIEQMQSLISDAEERGEQALQDAWQKLQDLEEALQQSKEELARLLRDYQAMLGVKLSLDVEIATYRQLLEGEESRMSGELQSHVSISVQNSQVSVNGGAGGGGSYGSGGYGGGSGGGYGGGRSYRGGGARGRSGGGYGSGCGGGGGSYGGSGRSGRGSSRVQIIQTSTNTSHRRILE |
| 预测分子量 | 63.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT77重组蛋白的模拟参考文献示例(请注意，以下内容为虚构示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: *Recombinant Expression and Functional Analysis of Keratin 77 in Epithelial Cell Differentiation*
**作者**: Zhang L, et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化了重组KRT77蛋白，验证了其在调节上皮细胞分化中的关键作用。实验表明，KRT77通过与细胞骨架蛋白的相互作用维持细胞机械稳定性，并可能参与皮肤屏障功能的调控。
2. **文献名称**: *KRT77 Recombinant Protein as a Biomarker in Colorectal Cancer Progression*
**作者**: Wang Y, et al.
**摘要**: 研究利用哺乳动物细胞表达体系制备了KRT77重组蛋白，发现其在结直肠癌组织中高表达。体外实验证实KRT77通过激活Wnt/β-catenin信号通路促进肿瘤细胞迁移，提示其作为潜在治疗靶点的可能性。
3. **文献名称**: *Structural Characterization of Human Keratin 77 and Its Role in Inflammatory Skin Disorders*
**作者**: Müller S, et al.
**摘要**: 通过杆状病毒-昆虫细胞系统获得高纯度KRT77重组蛋白，解析了其晶体结构。功能研究表明，KRT77在银屑病等炎症性皮肤病中异常表达，可能通过调控NF-κB通路加剧炎症反应。
4. **文献名称**: *Development of a Keratin 77-Specific Monoclonal Antibody Using Recombinant Protein Antigen*
**作者**: Kim H, et al.
**摘要**: 本研究通过原核表达KRT77重组蛋白片段，成功制备了特异性单克隆抗体。该抗体在免疫组化中显示出对KRT77的高灵敏度，为临床病理诊断提供了新工具。
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**注意事项**：
- 以上文献为示例模板，实际研究请通过 **PubMed(https://pubmed.ncbi.nlm.nih.gov)** 或 **Google Scholar** 检索。
- 若需真实文献，建议使用关键词 `"KRT77 recombinant"` 或 `"Keratin 77 protein expression"` 进行搜索，并筛选近5年的研究以获取最新进展。
Keratins are a diverse family of structural proteins that form intermediate filaments, providing mechanical support and regulatory functions in epithelial cells. KRT77. also known as keratin 77. is a type I keratin encoded by the *KRT77* gene. It is primarily expressed in stratified and glandular epithelia, playing roles in maintaining cellular integrity, stress response, and tissue-specific differentiation. Dysregulation of KRT77 has been implicated in pathological conditions, including inflammatory diseases, fibrosis, and cancer, making it a potential biomarker or therapeutic target.
Recombinant KRT77 protein is produced using biotechnological systems, such as *E. coli* or mammalian cell expression platforms, to generate purified, functional protein for research. Its production often involves cloning the *KRT77* gene into expression vectors, followed by induction, extraction, and purification via chromatography. Recombinant KRT77 retains structural and biochemical properties of the native protein, enabling studies on its interactions, post-translational modifications, and role in cellular processes like apoptosis, migration, and signaling.
Research applications include investigating keratin-mediated diseases, such as skin disorders or epithelial cancers, where KRT77 overexpression or mutation may drive pathology. It is also used to develop antibodies for diagnostic assays or to explore its utility in tissue engineering. Challenges in working with recombinant KRT77 include its insolubility due to hydrophobic domains and the need to mimic native phosphorylation or glycosylation patterns. Current studies focus on optimizing expression systems and deciphering its context-dependent functions in health and disease.
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