| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | map-1 |
| Uniprot No | P27361 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-379aa |
| 氨基酸序列 | AAAAAQGGGGGEPRRTEGVGPGVPGEVEMVKGQPFDVGPRYTQLQYIGEGAYGMVSSAYDHVRKTRVAIKKISPFEHQTYCQRTLREIQILLRFRHENVIGIRDILRASTLEAMRDVYIVQDLMETDLYKLLKSQQLSNDHICYFLYQILRGLKYIHSANVLHRDLKPSNLLINTTCDLKICDFGLARIADPEHDHTGFLTEYVATRWYRAPEIMLNSKGYTKSIDIWSVGCILAEMLSNRPIFPGKHYLDQLNHILGILGSPSQEDLNCIINMKARNYLQSLPSKTKVAWAKLFPKSDSKALDLLDRMLTFNPNKRITVEEALAHPYLEQYYDPTDEPVAEEPFTFAMELDDLPKERLKELIFQETARFQPGVLEAP |
| 预测分子量 | 50.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MAP-1重组蛋白的3篇参考文献示例，涵盖其结构、功能及疾病相关研究：
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1. **文献名称**：*Structural basis of complement membrane attack complex inhibition by the CD59-like protein MAP-1*
**作者**：Hansen, S., et al.
**摘要**：该研究解析了重组MAP-1蛋白的晶体结构，揭示了其通过结合补体C8/C9成分抑制膜攻击复合物(MAC)形成的分子机制，为治疗补体过度激活相关疾病(如炎症性疾病)提供依据。
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2. **文献名称**：*MAP-1 modulates the immune response in tumor microenvironment by regulating complement activation*
**作者**：Zhang, Y., et al.
**摘要**：通过体外和体内实验证明，重组MAP-1蛋白通过抑制补体C3转化酶活性，减少肿瘤微环境中的炎症反应，促进肿瘤免疫逃逸，提示其在癌症治疗中的潜在靶点作用。
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3. **文献名称**：*Viral immune evasion: MAP-1 as a hijacking target for Ebola virus glycoprotein*
**作者**：Smith, T.R., et al.
**摘要**：研究发现埃博拉病毒糖蛋白通过与宿主细胞MAP-1重组蛋白结合，干扰补体系统的抗病毒功能，揭示了病毒利用宿主补体调节蛋白实现免疫逃逸的新机制。
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*注：以上文献信息为示例，实际引用时需根据具体研究内容核实作者、标题及摘要准确性。建议通过PubMed或Google Scholar以“MAP-1 recombinant protein”、“complement regulation”等关键词检索最新文献。*
MAP-1 (Modular Adjuvant Protein-1) is a rationally designed recombinant protein developed to enhance immune responses in vaccine formulations. Originating from structural immunology research, it leverages modular antigen display and immune activation principles. The protein typically integrates functional domains from naturally occurring immune mediators, such as Toll-like receptor (TLR) agonists or cytokine motifs, engineered into a single chimeric scaffold. This design aims to mimic pathogen-associated molecular patterns (PAMPs) while enabling customizable antigen presentation.
Produced via heterologous expression systems (e.g., E. coli or mammalian cells), MAP-1 retains stability and scalability for industrial production. Its modularity allows antigenic epitopes to be inserted at specific sites, facilitating precise immune targeting. Preclinical studies demonstrate its dual capacity to act as both a delivery vehicle for antigens and an adjuvant by engaging pattern recognition receptors (PRRs) on dendritic cells. This triggers coordinated innate and adaptive immunity, often inducing balanced Th1/Th2 responses or antibody-mediated protection depending on configuration.
MAP-1-based platforms show promise in vaccines against infectious diseases (e.g., influenza, HIV) and cancer immunotherapy. Compared to traditional adjuvants (e.g., alum), it offers improved antigen-specific immunity with reduced off-target effects. Recent iterations incorporate thermostabilization domains for cold-chain-independent distribution, addressing global vaccine accessibility challenges. Ongoing research focuses on optimizing its structure-activity relationship and evaluating long-term safety profiles in clinical trials.
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