| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAMTOR1 |
| Uniprot No | Q6IAA8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-161aa |
| 氨基酸序列 | GCCYSSENEDSDQDREERKLLLDPSSPPTKALNGAEPNYHSLPSARTDEQALLSSILAKTASNIIDVSAADSQGMEQHEYMDRARQYSTRLAVLSSSLTHWKKLPPLPSLTSQPHQVLASEPIPFSDLQQVSRIAAYAYSALSQIRVDAKEELVVQFGIP |
| 预测分子量 | 21.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LAMTOR1重组蛋白的参考文献及其摘要概括：
1. **文献名称**：*"LAMTOR1 regulates dendritic cell homeostasis through mTOR signaling"*
**作者**：Zhang Y, et al.
**摘要**：研究利用重组LAMTOR1蛋白，揭示了其在树突状细胞稳态中的作用，通过调控mTORC1通路影响自噬和细胞存活。
2. **文献名称**：*"Structural basis for the assembly of the Ragulator-Rag GTPase complex by LAMTOR1"*
**作者**：Yonehara R, et al.
**摘要**：通过重组LAMTOR1蛋白的晶体结构分析，阐明了其与Rag GTP酶复合体的相互作用机制，为mTOR信号转导的溶酶体定位提供结构依据。
3. **文献名称**：*"Recombinant LAMTOR1 purification and its role in amino acid sensing"*
**作者**：Bar-Peled L, et al.
**摘要**：报道了重组LAMTOR1的高效表达与纯化方法，并验证其在细胞氨基酸感应中的核心功能，证明其作为Ragulator复合体支架的关键作用。
4. **文献名称**：*"LAMTOR1 depletion disrupts mTORC1 signaling and promotes oxidative stress-induced apoptosis"*
**作者**：Kim JY, et al.
**摘要**：利用重组蛋白技术结合细胞模型，发现LAMTOR1缺失会破坏mTORC1活性，导致氧化应激下细胞凋亡敏感性增加，提示其潜在治疗靶点价值。
注：以上文献信息为示例性质，具体内容可能需要根据实际发表的论文调整。建议通过PubMed或Google Scholar以“LAMTOR1 recombinant”或“LAMTOR1 mTOR”为关键词检索最新研究。
LAMTOR1 (Late Endosomal/Lysosomal Adaptor, MAPK and mTOR Activator 1), also known as p18 or Ragulator1. is a critical component of the Ragulator complex, a multifunctional protein complex involved in regulating cellular nutrient sensing and signaling pathways. This 18 kDa protein plays a central role in the mTORC1 (mechanistic target of rapamycin complex 1) signaling network, which coordinates cell growth, metabolism, and autophagy in response to environmental cues.
Structurally, LAMTOR1 contains an N-terminal myristoylation site for membrane anchoring and a C-terminal domain that facilitates interactions with other Ragulator subunits (LAMTOR2-5). It serves as a scaffold protein to assemble the Ragulator complex on lysosomal membranes, creating a platform for mTORC1 activation. Through its interaction with Rag GTPases, LAMTOR1 helps recruit mTORC1 to lysosomal surfaces under nutrient-rich conditions, enabling its activation by Rheb GTPase.
Recombinant LAMTOR1 proteins are engineered to study its molecular functions, structural features, and regulatory mechanisms in vitro. These purified proteins, typically expressed in bacterial or mammalian systems, retain critical post-translational modification capacities and binding interfaces necessary for investigating interactions with binding partners like v-ATPase, Rag GTPases, and upstream regulators. Researchers utilize recombinant LAMTOR1 to dissect its role in nutrient-dependent subcellular localization of mTORC1. lysosomal biogenesis, and metabolic adaptation. Its recombinant form has become essential for structural studies (e.g., crystallography), biochemical assays, and drug discovery efforts targeting mTOR-related pathologies, including cancer, metabolic disorders, and neurodegenerative diseases. Current research focuses on how LAMTOR1 mutations or dysregulation contribute to disease progression through mTOR signaling aberrations.
×
