| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RASSF6 |
| Uniprot No | Q6ZTQ3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-303aa |
| 氨基酸序列 | MTMMAHQYPSWIFINEKTFITREQLNSLLKTYNIFYENQKNLHILYGETEDGKLIVEGMLDIFWGVKRPIQLKIQDEKPFSSFTSMKSSDVFSSKGMTRWGEFDDLYRISELDRTQIPMSEKRNSQEDYLSYHSNTLKPHAKDEPDSPVLYRTMSEAALVRKRMKPLMMDRKERQKNRASINGHFYNHEIENSPQDFALHIIFATGEQRRLKKTDIPLLQRLLQGPSEKNARIFLMDKDAEEISSDVAQYINFHFSLLESILQRLNEEEKREIQRIVTKFNKEKAIILKCLQNKLVIKTETTV |
| 预测分子量 | 62.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RASSF6重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
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1. **标题**: *"Recombinant RASSF6 Protein Induces Apoptosis via the Hippo Signaling Pathway"*
**作者**: Smith A, et al.
**摘要**: 研究利用大肠杆菌表达系统成功纯化RASSF6重组蛋白，并发现其通过激活Hippo通路核心激酶MST1.促进肿瘤细胞凋亡，揭示了其潜在的抑癌机制。
2. **标题**: *"Structural Characterization of RASSF6 and Its Interaction with Raf-1 Proto-Oncogene"*
**作者**: Chen L, et al.
**摘要**: 通过X射线晶体学解析RASSF6重组蛋白的SARAH结构域三维结构，证实其与Raf-1蛋白的直接结合，为RASSF6调控MAPK信号通路提供结构基础。
3. **标题**: *"RASSF6 Recombinant Protein Suppresses NSCLC Metastasis by Modulating EMT Markers"*
**作者**: Tanaka K, et al.
**摘要**: 在非小细胞肺癌模型中，外源性RASSF6重组蛋白显著抑制上皮-间质转化(EMT)，降低迁移能力，提示其作为转移抑制因子的治疗潜力。
4. **标题**: *"Development of a RASSF6-Based Biosensor for Real-Time Detection of Oxidative Stress"*
**作者**: Müller R, et al.
**摘要**: 研究利用RASSF6重组蛋白对活性氧(ROS)的敏感性，构建新型生物传感器，实现细胞氧化应激水平的动态监测。
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**备注**：以上文献为虚拟示例，实际研究中建议通过PubMed、Google Scholar等平台检索关键词“RASSF6 recombinant protein”“RASSF6 structure/function”获取真实文献。
**Background of RASSF6 Recombinant Protein**
RASSF6 (Ras association domain-containing family member 6) is a member of the RASSF tumor suppressor family, which is implicated in regulating cellular processes such as apoptosis, cell cycle arrest, and microtubule stability. Unlike classical Ras effectors, RASSF proteins lack enzymatic activity but serve as adaptors, modulating signaling pathways like Hippo, p53. and NF-κB. RASSF6 contains a Ras association (RA) domain and a SARAH (Sav-RASSF-Hippo) domain, enabling interactions with kinases such as MST1/2 to activate pro-apoptotic pathways.
RASSF6 expression is often downregulated in cancers (e.g., lung, colorectal, and breast tumors) due to promoter hypermethylation, correlating with poor prognosis. Its tumor-suppressive role involves inducing apoptosis via caspase activation, inhibiting cyclin D1 to block cell cycle progression, and suppressing oncogenic signaling. Beyond cancer, RASSF6 is linked to cardiovascular and immune regulation, highlighting its diverse physiological roles.
Recombinant RASSF6 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for *in vitro* studies. This tool enables exploration of RASSF6’s molecular interactions, post-translational modifications, and mechanisms in disease contexts. Researchers utilize it to identify binding partners, validate signaling pathways, and screen therapeutic compounds targeting RASSF6-related disorders. Challenges include maintaining proper folding of its RA and SARAH domains, critical for functionality.
Overall, RASSF6 recombinant protein is vital for deciphering its dual role in tumor suppression and tissue homeostasis, offering potential for diagnostic or therapeutic advancements in oncology and beyond.
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