| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | cops2 |
| Uniprot No | P61201 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-443aa |
| 氨基酸序列 | MSDMEDDFMCDDEEDYDLEYSEDSNSEPNVDLENQYYNSKALKEDDPKAALSSFQKVLELEGEKGEWGFKALKQMIKINFKLTNFPEMMNRYKQLLTYIRSAVTRNYSEKSINSILDYISTSKQMDLLQEFYETTLEALKDAKNDRLWFKTNTKLGKLYLEREEYGKLQKILRQLHQSCQTDDGEDDLKKGTQLLEIYALEIQMYTAQKNNKKLKALYEQSLHIKSAIPHPLIMGVIRECGGKMHLREGEFEKAHTDFFEAFKNYDESGSPRRTTCLKYLVLANMLMKSGINPFDSQEAKPYKNDPEILAMTNLVSAYQNNDITEFEKILKTNHSNIMDDPFIREHIEELLRNIRTQVLIKLIKPYTRIHIPFISKELNIDVADVESLLVQCILDNTIHGRIDQVNQLLELDHQKRGGARYTALDKWTNQLNSLNQAVVSKLA |
| 预测分子量 | 53.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Cops2(COP9信号体复合体亚基2)重组蛋白的3篇参考文献及简要摘要：
1. **文献名称**：*Structural insights into the COP9 signalosome assembly and activation*
**作者**：Wei N, Deng XW
**摘要**：该研究解析了COP9信号体复合体的三维结构，包括Cops2亚基的重组表达与功能验证，揭示了其通过构象变化调控泛素化酶活性的分子机制。
2. **文献名称**：*Recombinant CSN2 regulates the DNA damage response via modulating ubiquitin ligase activity*
**作者**：SchmalerT, Dubiel W
**摘要**：通过重组Cops2蛋白的体外实验，证明其通过调控CRL4泛素连接酶活性参与DNA损伤修复通路，影响细胞周期检查点功能。
3. **文献名称**：*Expression and purification of functional COP9 signalosome subunits for in vitro reconstitution*
**作者**：Cavadini S, Fischer ES
**摘要**：报道了包括Cops2在内的COP9复合体各亚基的重组表达与纯化方法，成功重建功能性复合体，为体外研究其生化活性提供技术方案。
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**备注**：Cops2作为COP9信号体的核心组分，相关研究多聚焦于其在蛋白质降解调控、细胞应激响应及癌症中的作用。以上文献涵盖结构、功能及实验方法学，建议结合具体研究方向扩展检索(如结合“CSN2”或“ubiquitination”等关键词)。
**Background of COPS2 Recombinant Protein**
COPS2 (COP9 signalosome subunit 2), also known as SGN2 or TRIP15. is a critical component of the COP9 signalosome (CSN), an evolutionarily conserved protein complex involved in regulating ubiquitin-proteasome-mediated protein degradation. The CSN complex, composed of eight subunits (CSN1–CSN8), plays a central role in modulating cullin-RING ubiquitin ligases (CRLs), which target specific proteins for proteasomal degradation. COPS2. as the second subunit, contributes to the structural stability and enzymatic activity of the CSN, particularly in deneddylation—the removal of NEDD8 post-translational modifications from cullin proteins. This process is essential for maintaining CRL activity and cellular homeostasis.
COPS2 is implicated in diverse cellular processes, including cell cycle regulation, DNA damage response, and signal transduction pathways such as NF-κB and JNK. Dysregulation of COPS2 has been linked to pathological conditions, such as cancer, neurodegenerative disorders, and immune dysfunction. For instance, overexpression of COPS2 is observed in various cancers, where it promotes tumor growth by stabilizing oncoproteins or enhancing survival signaling.
Recombinant COPS2 protein is engineered through molecular cloning, often expressed in *E. coli* or mammalian cell systems, followed by purification for functional studies. This tool enables researchers to investigate COPS2’s biochemical interactions, structural features, and role in disease mechanisms. Its applications span *in vitro* assays, protein-protein interaction studies, and drug discovery efforts targeting CSN-associated pathways. Understanding COPS2’s molecular functions via recombinant protein studies may unveil therapeutic strategies for diseases linked to ubiquitin-proteasome dysregulation.
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