| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LYZL1 |
| Uniprot No | Q6UWQ5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-194aa |
| 氨基酸序列 | MQDAPLSCLSPTKWSSVSSADSTEKSASGAGTRNLPFQFCLRQALRMKAAGILTLIGCLVTGAESKIYTRCKLAKIFSRAGLDNYWGFSLGNWICMAYYESGYNTTAQTVLDDGSIDYGIFQINSFAWCRRGKLKENNHCHVACSALITDDLTDAIICARKIVKETQGMNYWQGWKKHCEGRDLSEWKKGCEVS |
| 预测分子量 | 37.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LYZL1重组蛋白的示例参考文献(内容基于领域内常见研究方向，具体文献需通过学术数据库核实)：
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1. **文献名称**：*LYZL1. a novel human recombinantly expressed protein, exhibits lysozyme-like activity in sperm maturation*
**作者**：Zhang Y. et al.
**摘要**：研究通过重组表达技术获得LYZL1蛋白，证实其在体外具有类似溶菌酶的酶活性，并揭示了其通过水解肽聚糖参与精子表面修饰的机制，提示LYZL1可能影响男性生殖功能。
2. **文献名称**：*Structural characterization of recombinant LYZL1 and its potential role in innate immunity*
**作者**：Chen L. & Wang X.
**摘要**：通过X射线晶体学解析LYZL1的三维结构，发现其与经典溶菌酶结构域的相似性。功能实验表明，重组LYZL1对革兰氏阳性菌具有抑菌作用，可能参与黏膜免疫防御。
3. **文献名称**：*Downregulation of LYZL1 in asthenozoospermia: Implications for male infertility diagnostics*
**作者**：Liu R. et al.
**摘要**：临床研究发现弱精症患者精液中LYZL1表达显著降低。利用重组LYZL1蛋白开发检测试剂，提示其可作为男性不育的生物标志物。
4. **文献名称**：*Recombinant LYZL1 interaction with zona pellucida glycoproteins during fertilization*
**作者**：Kim S. et al.
**摘要**：通过体外结合实验证实重组LYZL1能与卵母细胞透明带蛋白(如ZP2)相互作用，推测其在精子-卵子识别中起调控作用，为辅助生殖技术提供新靶点。
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**建议**：
- 以上为模拟示例，实际文献请通过 **PubMed、Web of Science 或 Google Scholar** 检索关键词“LYZL1 recombinant protein”“LYZL1 function”等获取。
- 部分真实研究可能集中在LYZL家族(如LYZL6)或与其他溶菌酶样蛋白的对比分析，建议拓展检索范围。
LYZL1 (Lysozyme-like protein 1) is a member of the lysozyme family, a group of proteins traditionally recognized for their antimicrobial activity through peptidoglycan hydrolysis. While lysozymes are widely studied in innate immunity, LYZL1 has distinct biological roles, particularly in reproductive biology. It was first identified in humans as a testis-specific protein, with subsequent studies revealing its expression in the male reproductive tract, including the epididymis and seminal plasma. Structurally, LYZL1 shares conserved features with c-type lysozymes, such as a glycoside hydrolase domain, but lacks classical muramidase activity, suggesting functional divergence during evolution.
Research highlights LYZL1’s involvement in sperm maturation and fertilization. It is secreted by the epididymis and binds to sperm surfaces, potentially modulating sperm motility, capacitation, or zona pellucida interaction. Animal studies in mice and rats support its role in fertility, as LYZL1 knockout models exhibit impaired sperm function. Beyond reproduction, LYZL1 has been detected in certain cancers, including prostate and breast malignancies, though its oncogenic or tumor-suppressive mechanisms remain unclear.
Recombinant LYZL1 protein, typically produced in bacterial or mammalian expression systems, enables functional studies and therapeutic exploration. Its applications span in vitro fertilization research, male infertility diagnostics, and drug development targeting reproductive disorders. Recent advances in structural biology and proteomics continue to unravel its molecular interactions, offering insights into its dual roles in reproduction and disease. Despite progress, questions persist about its precise molecular targets and regulatory pathways, driving ongoing investigations into its therapeutic potential.
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