| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Ctdp1 |
| Uniprot No | Q9Y5B0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-961aa |
| 氨基酸序列 | MEVPAAGRVPAEGAPTAAVAEVRCPGPAPLRLLEWRVAAGAAVRIGSVLAVFEAAASAQSSGASQSRVASGGCVRPARPERRLRSERAGVVRELCAQPGQVVAPGAVLVRLEGCSHPVVMKGLCAECGQDLTQLQSKNGKQQVPLSTATVSMVHSVPELMVSSEQAEQLGREDQQRLHRNRKLVLMVDLDQTLIHTTEQHCQQMSNKGIFHFQLGRGEPMLHTRLRPHCKDFLEKIAKLYELHVFTFGSRLYAHTIAGFLDPEKKLFSHRILSRDECIDPFSKTGNLRNLFPCGDSMVCIIDDREDVWKFAPNLITVKKYVYFQGTGDMNAPPGSRESQTRKKVNHSRGTEVSEPSPPVRDPEGVTQAPGVEPSNGLEKPARELNGSEAATPRDSPRPGKPDERDIWPPAQAPTSSQELAGAPEPQGSCAQGGRVAPGQRPAQGATGTDLDFDLSSDSESSSESEGTKSSSSASDGESEGKRGRQKPKAAPEGAGALAQGSSLEPGRPAAPSLPGEAEPGAHAPDKEPELGGQEEGERDGLCGLGNGCADRKEAETESQNSELSGVTAGESLDQSMEEEEEEDTDEDDHLIYLEEILVRVHTDYYAKYDRYLNKEIEEAPDIRKIVPELKSKVLADVAIIFSGLHPTNFPIEKTREHYHATALGAKILTRLVLSPDAPDRATHLIAARAGTEKVLQAQECGHLHVVNPDWLWSCLERWDKVEEQLFPLRDDHTKAQRENSPAAFPDREGVPPTALFHPMPVLPKAQPGPEVRIYDSNTGKLIRTGARGPPAPSSSLPIRQEPSSFRAVPPPQPQMFGEELPDAQDGEQPGPSRRKRQPSMSETMPLYTLCKEDLESMDKEVDDILGEGSDDSDSEKRRPEEQEEEPQPRKPGTRRERTLGAPASSERSAAGGRGPRGHKRKLNEEDAASESSRESSNEDEGSSSEADEMAKALEAELNDLM |
| 预测分子量 | 104,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CTDP1重组蛋白的模拟参考文献示例(仅供参考，具体文献需通过学术数据库查询)：
1. **文献名称**: "Expression and Functional Characterization of Recombinant Human CTDP1 in E. coli"
**作者**: Smith A, et al.
**摘要**: 报道了在大肠杆菌中成功表达并纯化重组人CTDP1蛋白，证实其具有RNA聚合酶II C端结构域(CTD)的去磷酸化活性，为后续功能研究奠定基础。
2. **文献名称**: "CTDP1 Mutations and Impaired Phosphatase Activity in Neurodevelopmental Disorders"
**作者**: Johnson R, et al.
**摘要**: 通过重组CTDP1蛋白的酶动力学实验，发现致病突变体导致磷酸酶活性显著降低，提示其与先天性小脑发育不全的分子机制相关。
3. **文献名称**: "Structural Insights into CTDP1 Regulation by Oligomerization"
**作者**: Lee H, et al.
**摘要**: 利用重组CTDP1的晶体结构分析，揭示其寡聚化状态对磷酸酶活性的调控作用，为靶向治疗策略提供结构基础。
4. **文献名称**: "Development of a CTDP1 Knockout Cell Model Using Recombinant Protein Complementation"
**作者**: Wang Y, et al.
**摘要**: 在CTDP1缺陷细胞中导入重组蛋白，验证其恢复CTD去磷酸化过程的能力，证实其在转录调控中的关键功能。
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**提示**：实际文献建议通过PubMed、Web of Science等平台检索关键词：
`CTDP1 recombinant protein expression`、`CTDP1 phosphatase activity`、`CTDP1 structural analysis`。
CTDP1 (C-terminal domain phosphatase 1) is a protein encoded by the CTDP1 gene in humans, primarily recognized for its role as a serine/threonine phosphatase regulating transcription and cell cycle progression. It specifically dephosphorylates the C-terminal domain (CTD) of RNA polymerase II (Pol II), a critical step in transitioning from transcription initiation to elongation. This activity links CTDP1 to the regulation of gene expression, DNA repair, and mRNA processing. Structurally, CTDP1 contains an N-terminal phosphatase domain and a C-terminal regulatory region, which mediates protein-protein interactions and subcellular localization.
Mutations in CTDP1 are associated with congenital cataracts, facial dysmorphism, and neuropathy (CCFDN syndrome), a rare autosomal recessive disorder, underscoring its importance in neural and ocular development. Additionally, aberrant CTDP1 expression has been implicated in cancer, where its phosphatase activity may influence oncogenic signaling pathways, such as PI3K/AKT/mTOR.
Recombinant CTDP1 protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties, substrate specificity, and interactions. Its recombinant form enables in vitro assays to explore enzymatic kinetics, screen inhibitors, or model CTD dephosphorylation mechanisms. Researchers also utilize it to investigate molecular defects in CCFDN syndrome or its role in transcription-coupled DNA repair. The availability of purified CTDP1 recombinant protein supports structural studies (e.g., crystallography) and functional genomics, aiding therapeutic development for CTDP1-related diseases. Its study bridges fundamental transcription biology and clinical insights into developmental disorders and cancer.
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