Recombinant Human GPR15L protein

中文名： 蛋白质GPR15L(GPR15L)重组蛋白
别名： GPR15L；C10orf99；GPR15L；Protein GPR15LG

货号: PA2000-3167

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	GPR15L
Uniprot No	Q6UWK7
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	25-81aa
氨基酸序列	KRRPAKAWSGRRTRLCCHRVPSPNSTNLKGHHVRLCKPCKLEPEPRLWVVPGALPQV
预测分子量	14.0 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于GPR15L重组蛋白的模拟参考文献示例(实际文献可能存在差异，建议通过学术数据库核实)：

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1. **Title**: *Recombinant GPR15L Protein Induces Chemotaxis of CD4+ T Cells via GPR15 Activation*

**Authors**: Smith A, Lee B, et al.

**Summary**: 该研究通过原核表达系统成功制备重组GPR15L蛋白，证实其能特异性结合GPR15受体，并促进CD4+ T细胞在体外迁移实验中的趋化性，提示GPR15L/GPR15轴在肠道免疫细胞归巢中的潜在作用。

2. **Title**: *Structural and Functional Characterization of Human GPR15L Expressed in Mammalian Cells*

**Authors**: Zhang Y, Wang H, et al.

**Summary**: 利用哺乳动物HEK293细胞系统表达并纯化重组人源GPR15L蛋白，结合晶体结构分析和信号通路检测，揭示了其C末端结构域对GPR15受体激活的关键性，为开发靶向药物奠定基础。

3. **Title**: *GPR15L as a Novel Mucosal Chemokine: Role in Colitis Mouse Models*

**Authors**: Tanaka K, et al.

**Summary**: 研究通过重组GPR15L蛋白处理小鼠结肠炎模型，发现其加剧Th17细胞浸润和炎症反应，证明GPR15L在肠道黏膜免疫中的促炎功能，可能成为炎症性肠病治疗靶点。

4. **Title**: *Expression and Purification of Biologically Active GPR15L in a Baculovirus System*

**Authors**: Müller R, et al.

**Summary**: 描述利用杆状病毒-昆虫细胞系统高效表达具有生物活性的GPR15L重组蛋白，优化纯化工艺并通过体外功能实验验证其诱导T细胞迁移的能力，为大规模生产提供方法学支持。

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**注意事项**：

- 上述文献为示例性质，具体内容需以实际发表论文为准。

- 建议通过PubMed、Web of Science等平台搜索关键词“GPR15L recombinant protein”获取最新研究。

- GPR15L相关研究常与免疫调节、炎症疾病及受体信号机制关联，可进一步关注其与HIV感染或肠道屏障功能的交叉研究。

背景信息

**Background of GPR15L Recombinant Protein**

GPR15L (G Protein-Coupled Receptor 15 Ligand) is a recently identified protein that serves as the endogenous ligand for the orphan receptor GPR15. a class A G protein-coupled receptor (GPCR). Initially discovered through bioinformatic and functional screening approaches, GPR15L is encoded by the *C10orf99* gene in humans and shares structural homology with prokineticin family proteins, though it lacks their conserved N-terminal sequence. This secreted glycoprotein plays a critical role in regulating immune responses, particularly in T-cell trafficking and tissue-specific homing.

GPR15L binds to GPR15. which is highly expressed in immune cells, including regulatory T cells (Tregs) and effector lymphocytes, as well as in epithelial and mucosal tissues. The GPR15L-GPR15 axis is implicated in mucosal immunity, inflammation, and immune tolerance. For instance, it facilitates the homing of T cells to the colon, influencing inflammatory bowel disease (IBD) pathogenesis, and modulates skin immune responses. Dysregulation of this pathway has been linked to autoimmune disorders and cancer progression.

Recombinant GPR15L is produced using engineered host systems (e.g., mammalian cells or *E. coli*) to ensure proper post-translational modifications and bioactivity. It is purified via affinity chromatography, often with tags for enhanced stability. This protein is widely used in vitro and in vivo to study receptor-ligand interactions, signaling mechanisms (e.g., cAMP or MAPK pathways), and therapeutic targeting. Its applications extend to drug discovery for autoimmune diseases, colitis, and cancer immunotherapy.

Current research focuses on elucidating its structural determinants, tissue-specific functions, and potential as a biomarker or therapeutic target, highlighting its emerging significance in immunology and translational medicine.