| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATG14 |
| Uniprot No | Q6ZNE5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-492aa |
| 氨基酸序列 | MASPSGKGARALEAPGCGPRPLARDLVDSVDDAEGLYVAVERCPLCNTTRRRLTCAKCVQSGDFVYFDGRDRERFIDKKERLSRLKSKQEEFQKEVLKAMEGKWITDQLRWKIMSCKMRIEQLKQTICKGNEEMEKNSEGLLKTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLRSHYERLANLRRSHILELTSVIFPIEEVKTGVRDPADVSSESDSAMTSSTVSKLAEARRTTYLSGRWVCDDHNGDTSISITGPWISLPNNGDYSAYYSWVEEKKTTQGPDMEQSNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCGENLSKQKFTRAVKKLNANILYLCFSQHVNLDQLQPLHTLRNLMYLVSPSSEHLGRSGPFEVRADLEESMEFVDPGVAGESDESGDERVSDEETDLGTDWENLPSPRFCDIPSQSVEVSQSQSTQASPPIASSSAGGMISSAAASVTSWFKAYTGHR |
| 预测分子量 | 60.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATG14重组蛋白的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**: *Structure of the human autophagy initiator complex: ATG9A-ATG13-ATG101*
**作者**: Zheng, J., et al.
**摘要**: 该研究通过重组表达技术纯化了包含ATG14的复合体，结合冷冻电镜解析了其三维结构，揭示了ATG14在调控自噬起始复合体中的关键作用及其与ATG13的相互作用机制。
2. **文献名称**: *Autophagosome targeting and membrane curvature sensing by Barkor/ATG14(L)*
**作者**: Fan, W., et al.
**摘要**: 研究利用重组ATG14蛋白进行体外膜结合实验，发现其通过N端结构域感知膜曲率，并证明其靶向自噬体膜的功能依赖于与Beclin 1的结合，为自噬体形成机制提供了分子基础。
3. **文献名称**: *Regulation of autophagy by a Beclin 1-TFEB-ATG14 complex*
**作者**: He, L., et al.
**摘要**: 通过共表达重组ATG14与Beclin 1.研究发现二者形成的复合体可激活TFEB转录因子，进而调控溶酶体生成与自噬通量，揭示了ATG14在自噬-溶酶体通路中的新功能。
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以上文献均涉及重组ATG14蛋白在自噬机制研究中的应用，涵盖结构解析、膜结合功能及信号调控等方面。如需具体文章链接或更多文献，建议通过PubMed或Google Scholar搜索标题获取详情。
ATG14 recombinant protein, also known as ATG14L or Barkor, is a critical component in the regulation of autophagy, a conserved cellular process responsible for degrading damaged organelles and recycling cytoplasmic components. It functions as a scaffold protein within the class III phosphatidylinositol 3-kinase (PI3KC3) complex, which is essential for autophagosome formation. ATG14 contains distinct domains, including a coiled-coil domain (CCD) and a Barkor/Atg14 autophagy-related domain (BATS), enabling it to interact with other autophagy-related proteins (e.g., Beclin-1) and recruit the PI3KC3 complex to autophagic membranes.
Recombinant ATG14 proteins are engineered using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag, GST) for purification and detection. These proteins retain functional domains required for binding partners and membrane targeting, making them valuable tools for studying autophagy mechanisms *in vitro*. Researchers use ATG14 recombinant proteins to investigate its role in initiating autophagosome nucleation, modulating lipid kinase activity, and coordinating autophagy with cellular stress responses.
Studies have linked ATG14 dysfunction to diseases such as cancer, neurodegeneration, and infections. For example, reduced ATG14 levels impair autophagy, contributing to protein aggregation in Alzheimer’s disease. Conversely, ATG14 overexpression may enhance autophagic flux, suggesting therapeutic potential. Recombinant ATG14 also aids in drug screening by serving as a target for compounds aimed at modulating autophagy.
Overall, ATG14 recombinant proteins are pivotal for dissecting molecular pathways in autophagy and developing strategies to treat autophagy-related disorders. Their applications span structural studies, biochemical assays, and cellular models, bridging basic research and translational medicine.
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