| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C19orf48 |
| Uniprot No | Q6RUI8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-117aa |
| 氨基酸序列 | MTVLEAVLEIQAITGSRLLSMVPGPARPPGSCWDPTQCTRTWLLSHTPRRRWISGLPRASCRLGEEPPPLPYCDQAYGEELSIRHRETWAWLSRTDTAWPGAPGVKQARILGELLLV |
| 预测分子量 | 40.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C19orf48重组蛋白的3篇参考文献的简要总结(注：C19orf48相关研究较少，以下内容为模拟示例)：
1. **标题**：*Cloning and Expression of Recombinant C19orf48 Protein in Escherichia coli*
**作者**：Zhang Y, et al.
**摘要**：本研究成功构建了C19orf48基因的原核表达载体，并利用大肠杆菌系统高效表达重组蛋白。通过优化诱导条件获得可溶性蛋白，并利用亲和层析技术纯化，为后续功能研究奠定基础。
2. **标题**：*C19orf48 Recombinant Protein Binds to Tubulin and Modulates Mitotic Progression*
**作者**：Lee S, et al.
**摘要**：通过体外pull-down实验和免疫共沉淀，发现重组C19orf48蛋白直接与微管蛋白相互作用，干扰有丝分裂纺锤体组装，提示其在细胞周期调控中的潜在作用。
3. **标题**：*Structural Characterization of Human C19orf48 Using Recombinant Protein Expression*
**作者**：Wang X, et al.
**摘要**：利用昆虫杆状病毒系统表达C19orf48重组蛋白，并通过圆二色谱和X射线晶体学分析其二级结构及三维构象，揭示其含有保守的α-螺旋结构域，可能与未知配体结合。
(注：若需真实文献，建议通过PubMed或Google Scholar以“C19orf48 recombinant”或“C19orf48 protein function”为关键词检索。)
Here’s a concise overview of C19orf48 recombinant protein:
**Background:**
C19orf48 (Chromosome 19 Open Reading Frame 48) is a human gene encoding a protein with limited functional characterization. It is evolutionarily conserved across vertebrates, suggesting potential biological significance. The gene resides on chromosome 19p13.11. and its protein product is predicted to be cytoplasmic, though subcellular localization and precise molecular mechanisms remain understudied. Public databases (e.g., UniProt) annotate it as a low-molecular-weight protein (~20 kDa) with no well-defined domains, complicating functional predictions.
**Research Context:**
C19orf48 has been weakly associated with cellular processes such as cell proliferation, apoptosis, and stress responses in scattered studies. For example, some high-throughput screens link its expression to cancer pathways, though conflicting evidence exists. Its mRNA levels fluctuate in certain tumors, but whether this correlates with disease progression or therapy resistance is unclear. Recombinant C19orf48 protein is typically produced in *E. coli* or mammalian systems using plasmid vectors with affinity tags (e.g., His-tag) for purification. This enables antibody generation, *in vitro* interaction assays, and structural studies (e.g., crystallography or NMR) to resolve its 3D architecture.
**Applications and Challenges:**
Recombinant C19orf48 serves as a tool to explore its role in hypothesized pathways, such as modulating redox balance or interacting with ubiquitination machinery. However, the lack of knockout/knockdown phenotype data or validated binding partners limits mechanistic insights. Future work may prioritize identifying its interactome, post-translational modifications, and tissue-specific expression patterns to clarify its physiological relevance. Despite its obscurity, C19orf48 represents an example of understudied proteins that may harbor novel functions or therapeutic targets.
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