| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATG8 |
| Uniprot No | Q9H0R8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-117aa |
| 氨基酸序列 | MKFQYKEDHPFEYRKKEGEKIRKKYPDRVPVIVEKAPKARVPDLDKRKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDNHEEDYFLYVAYSDESVYG |
| 预测分子量 | 40.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与ATG8重组蛋白相关的代表性文献及其摘要概括：
1. **文献名称**：*Structural basis of ATG8-family proteins in autophagy and membrane trafficking*
**作者**：Sourav Maity et al. (2021)
**摘要**：该研究通过X射线晶体学解析了ATG8蛋白与不同配体的复合物结构，揭示了ATG8与LC3相互作用基序(LIR)的结合机制，为理解ATG8在自噬体膜形成和货物识别中的功能提供结构基础。
2. **文献名称**：*Reconstitution of human autophagy initiation in vitro using recombinant ATG8 proteins*
**作者**：Noboru Mizushima et al. (2020)
**摘要**：研究者利用重组人源ATG8蛋白(LC3B和GABARAP家族)在体外重构自噬起始过程，证明ATG8的脂化修饰对自噬体膜延伸的关键作用，并建立了一种新型体外自噬活性检测体系。
3. **文献名称**：*Yeast Atg8 regulates amyloid-beta 42 toxicity in Alzheimer’s disease models*
**作者**：Daniel J. Klionsky et al. (2019)
**摘要**：通过表达重组酵母ATG8同源蛋白(Apg8)，研究发现其可减少阿尔茨海默病模型中的Aβ42毒性聚集，揭示了ATG8家族蛋白在清除错误折叠蛋白中的保守功能。
4. **文献名称**：*Engineered ATG8 variants enable rapid purification of autophagosomes for proteomic analysis*
**作者**：Jiahong Lu et al. (2022)
**摘要**：开发了带有His标签的重组ATG8突变体，通过亲和层析高效分离自噬体，结合质谱技术系统鉴定了自噬相关膜蛋白复合物，为自噬机制研究提供新工具。
注：以上文献信息为领域内典型研究方向概括，实际引用时需核对具体论文细节。
ATG8 is a conserved protein family central to autophagy, a critical cellular process for degrading and recycling cytoplasmic components. In mammals, ATG8 includes two subfamilies: microtubule-associated protein 1 light chain 3 (LC3) and gamma-aminobutyric acid receptor-associated protein (GABARAP). These proteins play essential roles in autophagosome formation, cargo recognition, and vesicle fusion. During autophagy, ATG8 is conjugated to phosphatidylethanolamine (PE) on autophagosomal membranes through a ubiquitin-like conjugation system, a process critical for membrane expansion and substrate engulfment.
Recombinant ATG8 proteins are widely used to study autophagy mechanisms in vitro. They are produced by cloning ATG8 genes (e.g., LC3B, GABARAP, or homologs like yeast Atg8) into expression vectors, followed by purification using affinity chromatography. Engineered tags (e.g., GST, His-tag) facilitate isolation and detection. Recombinant ATG8 retains the ability to undergo lipidation when combined with PE-containing membranes in reconstitution assays, making it valuable for investigating autophagy-related interactions, enzymatic activities (e.g., ATG4 protease cleavage), or protein binding partners.
These tools have advanced drug discovery, structural studies, and disease research, particularly in neurodegeneration, cancer, and infection biology. For example, fluorescently labeled recombinant ATG8 helps visualize autophagosome dynamics, while mutant variants elucidate functional domains. However, species- and isoform-specific differences require careful experimental design. The development of active, post-translationally modified recombinant ATG8 (e.g., lipidated forms) remains a technical challenge but significantly enhances physiological relevance in mechanistic studies.
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