| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ATG1 |
| Uniprot No | O75385 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1050aa |
| 氨基酸序列 | MEPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKKNLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLADYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAGRRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGKADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPLRQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSGSSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSSCDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRTPSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSPTQFQTPRSSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRPYTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLGCRLHSAPNLSDLHVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRNLRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLARQGVVMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFSTSRLTDLLLKAAFGTQAPDPGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSPVVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPELPAPGHGCSFADPITANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTLLFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLVLYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQGLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQHREGCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGICA |
| 预测分子量 | 112,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ATG1重组蛋白的3篇代表性文献摘要信息：
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1. **文献名称**：*Structure of the Human Atg13-Atg1 HORMA Domain Complex and Its Role in mTORC1 Signaling*
**作者**：Yamaguchi M, et al.
**摘要**：本研究解析了人源ATG1(ULK1)与ATG13复合物的HORMA结构域晶体结构，揭示了其在自噬起始阶段通过mTORC1信号通路调控的分子机制。通过重组蛋白表达技术，验证了二者互作对自噬体形成的关键作用。
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2. **文献名称**：*Reconstitution of Atg1 Kinase Activity Using Purified Yeast Proteins*
**作者**：Kabeya Y, et al.
**摘要**：作者利用重组酵母ATG1蛋白及其结合蛋白ATG13、ATG17.在体外重构了自噬相关激酶活性体系，证实了营养缺乏条件下ATG1复合物的自磷酸化及其对下游自噬进程的调控。
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3. **文献名称**：*Regulation of Atg1 in Development and Disease*
**作者**：Alers S, et al.
**摘要**：综述了ATG1在哺乳动物中的生物学功能，重点讨论了重组ATG1蛋白在解析其激酶活性、底物特异性及在神经退行性疾病模型中的应用，强调其在自噬治疗靶点开发中的潜力。
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注：以上文献为示例性内容，实际引用时请核对原文准确性及作者信息。如需具体文献，建议通过PubMed或Web of Science以“ATG1 recombinant protein”或“ULK1 purification”等关键词检索。
**Background of ATG1 Recombinant Protein**
ATG1 (Autophagy-related protein 1), also known as ULK1 (Unc-51-like kinase 1) in mammals, is a serine/threonine kinase central to the initiation of autophagy, a conserved cellular process for degrading and recycling cytoplasmic components. Discovered initially in yeast, ATG1/ULK1 forms a core complex with regulatory subunits like ATG13. FIP200. and ATG17 (in yeast) or ATG101 (in mammals), which collectively sense nutrient deprivation or stress signals to trigger autophagosome formation.
The activity of ATG1/ULK1 is tightly regulated by upstream signaling pathways, particularly mTOR (mechanistic target of rapamycin) and AMP-activated protein kinase (AMPK). Under nutrient-rich conditions, mTOR phosphorylates and inhibits ULK1. suppressing autophagy. During starvation, mTOR inactivation and AMPK activation relieve this inhibition, enabling ULK1 to phosphorylate downstream targets, thereby driving phagophore assembly and autophagosome biogenesis.
Recombinant ATG1 proteins are produced using expression systems like *E. coli* or mammalian cell cultures, often fused with tags (e.g., GST, His-tag) to facilitate purification and detection. These proteins are vital tools for *in vitro* studies to dissect autophagy mechanisms, screen modulators, or analyze kinase-substrate interactions. Researchers also utilize ATG1 recombinant proteins to study dysregulated autophagy in diseases, including cancer, neurodegenerative disorders, and infections, where autophagy pathways are implicated in pathogenesis or therapeutic resistance.
Overall, ATG1 recombinant proteins serve as essential reagents for unraveling the molecular intricacies of autophagy and developing targeted therapies.
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