| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH3PXD2A |
| Uniprot No | Q5TCZ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 902-986aa |
| 氨基酸序列 | PDPSGKELDTVPAKGRQNEGKSDSLEKIERRVQALNTVNQSKKATPPIPSKPPGGFGKTSGTPAVKMRNGVRQVAVRPQSVFVSP |
| 预测分子量 | 14.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SH3PXD2A重组蛋白的3篇参考文献示例(基于模拟文献内容)：
1. **文献名称**：*SH3PXD2A promotes invadopodia formation and invasion in cancer cells through scaffold protein interactions*
**作者**：Smith J, et al.
**摘要**：该研究通过表达重组SH3PXD2A蛋白，揭示了其作为支架蛋白与TKS5、N-WASP等分子的相互作用机制，证明其在侵袭性伪足形成和癌细胞转移中的关键作用。
2. **文献名称**：*Recombinant SH3PXD2A production and functional analysis in extracellular matrix degradation*
**作者**：Lee H, et al.
**摘要**：作者利用大肠杆菌系统表达并纯化重组SH3PXD2A蛋白，发现其能增强金属蛋白酶(MMPs)的活性，促进细胞外基质降解，为靶向癌症治疗提供依据。
3. **文献名称**：*Structural insights into the SH3 domains of SH3PXD2A and their role in podosome assembly*
**作者**：Garcia R, et al.
**摘要**：通过重组SH3PXD2A蛋白的结构解析，阐明了其SH3结构域与结合配体的分子机制，并验证了其在细胞足体组装中的动态调控功能。
(注：以上文献为模拟示例，实际引用需以真实文献为准。)
SH3PXD2A (SH3 and PX Domain-Containing 2A), also known as TKS5. is a scaffold protein involved in regulating cellular processes such as invadopodia formation, cell migration, and extracellular matrix remodeling. It belongs to the TKS adaptor protein family, characterized by N-terminal Phox homology (PX) and Src homology 3 (SH3) domains. The PX domain enables membrane association by binding to phosphatidylinositol lipids, while SH3 domains mediate protein-protein interactions, particularly with partners like N-WASP, MMPs, or signaling kinases. SH3PXD2A plays a critical role in cancer cell invasion and metastasis by coordinating the assembly of invadopodia—actin-rich protrusions that degrade the extracellular matrix. Its expression is upregulated in multiple cancers, including breast, lung, and glioblastoma, correlating with poor prognosis.
Recombinant SH3PXD2A protein is engineered for in vitro studies to dissect its molecular functions. Produced in systems like E. coli or mammalian cells, it often includes affinity tags (e.g., His-tag) for purification. Researchers use it to investigate interactions with binding partners, structural dynamics, or enzymatic activities (e.g., MMP activation). It also serves as a tool for screening inhibitors targeting invadopodia-mediated invasion or validating SH3PXD2A's role in signaling pathways like EGFR or PDGFR. Mutational studies using recombinant protein help map functional domains or phosphorylation sites (e.g., tyrosine residues critical for Src kinase signaling). Beyond cancer, SH3PXD2A is studied in developmental processes and diseases involving tissue remodeling, such as atherosclerosis. Its recombinant form bridges mechanistic insights and therapeutic exploration, highlighting its dual role as a biomarker and a potential drug target.
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