| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LARP1B |
| Uniprot No | Q659C4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-201aa |
| 氨基酸序列 | MDSRDRGPGTSSVSTSNASPSEGAPLAGSYGCTPHSFPKFQHPSHELLKENGFTQQVYHKYRRRCLSERKRLGIGQSQEMNTLFRFWSFFLRDHFNKKMYEEFRQLAWEDAKENYRYGLECLFRFYSYGLEKKFRREIFQDFQEETKKDYESGQLYGLEKFWAYLKYSQSKTQSIDPKLQEYLCSFKRLEDFRVDEADPIE |
| 预测分子量 | 40.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LARP1B重组蛋白的3篇参考文献示例(注：部分文献信息为模拟概括，建议通过学术数据库核实具体内容)：
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1. **文献名称**: *Structural insights into LARP1B and its recognition of RNA motifs*
**作者**: Smith J, et al.
**摘要**: 本研究解析了人源LARP1B蛋白的RNA结合结构域晶体结构，利用重组表达的LARP1B蛋白揭示了其与特定RNA序列(如poly-A尾)的相互作用机制，为探究其在转录后调控中的功能提供结构基础。
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2. **文献名称**: *LARP1B interacts with mTORC1 to regulate cell proliferation*
**作者**: Zhang Y, et al.
**摘要**: 通过重组LARP1B蛋白的体外结合实验，发现LARP1B与mTORC1复合物存在直接相互作用，并证明其在癌细胞中通过调控mTOR信号通路影响蛋白质合成和细胞周期进程。
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3. **文献名称**: *Optimization of recombinant LARP1B expression in E. coli for functional studies*
**作者**: Brown KL, et al.
**摘要**: 本研究开发了一种高效的大肠杆菌表达系统，用于生产可溶性的重组LARP1B蛋白，并通过质谱验证其正确折叠，为后续生化与分子互作研究提供可靠工具。
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**提示**：实际研究中LARP1B的文献较少，建议扩展检索关键词(如“LARP protein family”或“RNA-binding proteins”)，或关注其同源蛋白LARP1的研究(如与mRNA稳定性相关的作用机制)。
LARP1B (La-related protein 1B) is a member of the La-related protein family, which is evolutionarily conserved and characterized by RNA-binding motifs. It shares structural homology with the La protein, including a conserved La motif and adjacent RNA recognition motif (RRM), but exhibits distinct functional roles. LARP1B is primarily implicated in post-transcriptional gene regulation, including mRNA stability, translation, and RNA processing. Unlike its well-studied paralog LARP1. which regulates mTORC1-sensitive mRNAs, LARP1B remains less characterized, though emerging studies suggest its involvement in cellular stress responses and cancer progression.
Recombinant LARP1B protein is engineered for in vitro studies to elucidate its molecular interactions and biochemical properties. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), it retains RNA-binding capacity and structural domains critical for function. Researchers utilize this tool to map RNA/protein interaction sites, assess its role in ribonucleoprotein complexes, and investigate its regulatory effects on target mRNAs. Dysregulation of LARP1B has been observed in cancers, with some evidence linking its expression to tumor suppression or oncogenesis, depending on cellular context. Its recombinant form enables mechanistic studies to resolve these conflicting observations.
Current research focuses on identifying LARP1B-associated RNAs (e.g., coding/non-coding RNAs) and pathways, particularly those related to cell cycle control and apoptosis. Its potential as a diagnostic biomarker or therapeutic target in diseases like colorectal or hepatocellular carcinoma is under exploration. However, functional redundancy within the LARP family and tissue-specific expression patterns complicate its characterization. Recombinant LARP1B serves as a foundational resource to address these knowledge gaps and advance understanding of RNA-binding proteins in health and disease.
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