| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SLC25A30 |
| Uniprot No | Q5SVS4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-291aa |
| 氨基酸序列 | MSALNWKPFVYGGLASITAECGTFPIDLTKTRLQIQGQTNDAKFKEIRYRGMLHALVRIGREEGLKALYSGIAPAMLRQASYGTIKIGTYQSLKRLFIERPEDETLPINVICGILSGVISSTIANPTDVLKIRMQAQSNTIQGGMIGNFMNIYQQEGTRGLWKGVSLTAQRAAIVVGVELPVYDITKKHLILSGLMGDTVYTHFLSSFTCGLAGALASNPVDVVRTRMMNQRVLRDGRCSGYTGTLDCLLQTWKNEGFFALYKGFWPNWLRLGPWNIIFFVTYEQLKKLDL |
| 预测分子量 | 48.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SLC25A30重组蛋白的3篇假设性参考文献示例(注：具体文献需根据实际数据库检索确认)：
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1. **文献名称**: *Functional characterization of recombinant human SLC25A30 as a mitochondrial carnitine transporter*
**作者**: Fiermonte G. et al.
**摘要**: 本研究通过重组表达SLC25A30蛋白，验证其在线粒体内膜中作为肉碱转运体的功能，并分析其底物特异性及动力学参数。
2. **文献名称**: *Structural insights into SLC25A30 by cryo-EM reveal its role in nucleotide transport*
**作者**: Wang L. et al.
**摘要**: 利用冷冻电镜技术解析了重组SLC25A30的蛋白结构，揭示了其核苷酸转运机制及关键氨基酸位点的作用。
3. **文献名称**: *SLC25A30 overexpression in cancer cells alters mitochondrial metabolism and drug resistance*
**作者**: Chen X. et al.
**摘要**: 通过重组蛋白实验证明SLC25A30在肿瘤细胞中的异常表达影响线粒体代谢流，可能通过调节腺苷酸转运增强化疗耐药性。
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**提示**：实际文献需通过PubMed、Web of Science等平台检索，建议使用关键词 `SLC25A30 recombinant expression` 或 `SLC25A30 functional analysis` 筛选近5年研究。
**Background of SLC25A30 Recombinant Protein**
SLC25A30 is a member of the solute carrier family 25 (SLC25), a group of mitochondrial carrier proteins responsible for transporting metabolites, nucleotides, and cofactors across the mitochondrial inner membrane. These proteins play critical roles in cellular energy metabolism, amino acid homeostasis, and redox balance. SLC25A30. also known as mitochondrial phosphate carrier protein 2 (MPCP2), is implicated in the transport of phosphate, a key substrate for ATP synthesis and nucleotide biosynthesis. Its activity supports mitochondrial function, particularly in tissues with high metabolic demands like the liver, heart, and skeletal muscle.
Recombinant SLC25A30 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for biochemical and structural studies. The recombinant form allows researchers to investigate its transport mechanisms, substrate specificity, and regulatory interactions in controlled settings. Studies suggest SLC25A30 may have unique transport properties compared to other mitochondrial phosphate carriers, potentially influencing metabolic flexibility and stress adaptation.
Dysregulation of SLC25A30 has been linked to metabolic disorders, mitochondrial diseases, and cancer, where altered mitochondrial metabolism is a hallmark. Its recombinant variant serves as a tool to explore therapeutic targeting of mitochondrial transport pathways, such as designing inhibitors or activators to modulate cellular energy balance. Additionally, structural analyses of recombinant SLC25A30 could reveal insights into its 3D conformation and binding sites, aiding drug discovery efforts.
Overall, SLC25A30 recombinant protein is a vital resource for advancing our understanding of mitochondrial biology and its implications in health and disease.
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