| 纯度 | >90%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | Lcn3 |
| Uniprot No | Q62471 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-182aa |
| 氨基酸序列 | QDSSFLAFNNGNFSGKWFLKALVSEDDIPINKVSPMLILVLNNGDIELSITHMIYDQCLEVTTILEKTDVPGQYLAFEGKTHLQVQLSSVKGHYMLYCDGEIEGMRFLMTQLIGRDPQENLEALEEFKVFTQIKGLVAENLVILEQMEKCEPESFYELPSRPSE |
| 预测分子量 | 34.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LCN3(Lipocalin 3)重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **《Recombinant LCN3 protein inhibits bacterial growth by iron sequestration》**
- 作者：Smith A, et al.
- 摘要：研究通过大肠杆菌表达系统成功纯化重组LCN3蛋白，发现其通过结合铁载体抑制病原菌增殖，揭示了其在先天免疫中的潜在作用。
2. **《LCN3重组蛋白调节脂肪细胞分化的功能研究》**
- 作者：Zhang Y, et al.
- 摘要：利用哺乳动物细胞表达重组LCN3.证明其通过调控PPARγ信号通路促进脂肪细胞分化，为代谢疾病研究提供新靶点。
3. **《Structural and functional characterization of recombinant human LCN3》**
- 作者：Johnson R, et al.
- 摘要：通过X射线晶体学解析重组人源LCN3的三维结构，结合体外实验证实其与视黄醇结合能力，暗示其在维生素A转运中的功能。
4. **《LCN3重组蛋白在神经炎症中的保护作用》**
- 作者：Lee S, et al.
- 摘要：在小鼠模型中注射重组LCN3.发现其通过抑制小胶质细胞活化减轻神经炎症，为神经退行性疾病治疗提供新思路。
(注：以上文献为虚构示例，实际研究需通过PubMed、Web of Science等平台检索。)
**Background of Recombinant LCN3 Protein**
Lipocalin-3 (LCN3), a member of the lipocalin protein family, is a small secreted glycoprotein characterized by a conserved eight-stranded β-barrel structure that facilitates binding to hydrophobic molecules, such as lipids, steroids, and retinoids. This structural motif enables LCN3 to participate in diverse biological processes, including lipid transport, immune modulation, and cellular signaling. The protein is encoded by the *LCN3* gene, which is expressed in various tissues, with notable levels in the liver, kidney, and immune cells.
LCN3 has garnered attention for its role in inflammation and cancer. It interacts with specific cell surface receptors and modulates pathways like NF-κB and MAPK, influencing apoptosis, proliferation, and immune responses. In cancer, LCN3 is implicated in tumor progression, metastasis, and drug resistance, potentially serving as a biomarker or therapeutic target. Its involvement in lipid metabolism also links it to metabolic disorders, such as obesity and atherosclerosis.
Recombinant LCN3 protein is produced using expression systems (e.g., *E. coli*, mammalian cells) to ensure proper folding and post-translational modifications. This engineered protein retains native functionality, enabling studies on its mechanistic roles *in vitro* and *in vivo*. Researchers utilize recombinant LCN3 to investigate ligand-receptor interactions, screen therapeutic agents, and develop diagnostic tools. Challenges in production include maintaining solubility and bioactivity, often addressed through codon optimization or fusion tags.
Overall, recombinant LCN3 serves as a vital tool for deciphering its physiological and pathological roles, bridging basic research with translational applications in oncology, immunology, and metabolic disease.
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