| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACBD5 |
| Uniprot No | Q5T8D3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-461aa |
| 氨基酸序列 | MFQFHAGSWESWCCCCLIPADRPWDRGQHWQLEMADTRSVHETRFEAAVKVIQSLPKNGSFQPTNEMMLKFYSFYKQATEGPCKLSRPGFWDPIGRYKWDAWSSLGDMTKEEAMIAYVEEMKKIIETMPMTEKVEELLRVIGPFYEIVEDKKSGRSSDITSVRLEKISKCLEDLGNVLTSTPNAKTVNGKAESSDSGAESEEEEAQEEVKGAEQSDNDKKMMKKSADHKNLEVIVTNGYDKDGFVQDIQNDIHASSSLNGRSTEEVKPIDENLGQTGKSAVCIHQDINDDHVEDVTGIQHLTSDSDSEVYCDSMEQFGQEESLDSFTSNNGPFQYYLGGHSSQPMENSGFREDIQVPPGNGNIGNMQVVAVEGKGEVKHGGEDGRNNSGAPHREKRGGETDEFSNVRRGRGHRMQHLSEGTKGRQVGSGGDGERWGSDRGSRGSLNEQIALVLMRLQEDMQ |
| 预测分子量 | 67.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACBD5重组蛋白的模拟参考文献示例(注：以下内容为假设性示例，非真实文献)：
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1. **标题**: *Recombinant ACBD5 Facilitates Peroxisome-ER Membrane Tethering in Mammalian Cells*
**作者**: Smith A, et al.
**摘要**: 本研究利用哺乳动物表达系统成功重组表达了ACBD5蛋白，证实其通过结合过氧化物酶体膜蛋白PEX19和内质网锚定蛋白VAPB，调控细胞器间膜接触及脂质转运功能。
2. **标题**: *Structural and Functional Analysis of ACBD5 in Cholesterol Trafficking*
**作者**: Johnson B, et al.
**摘要**: 通过大肠杆菌表达系统获得高纯度ACBD5重组蛋白，结合X射线晶体学分析其酰基-CoA结合结构域，揭示其参与胆固醇代谢与细胞内运输的分子机制。
3. **标题**: *ACBD5 Knockdown and Recombinant Rescue in Neurodegenerative Disease Models*
**作者**: Lee C, et al.
**摘要**: 构建ACBD5重组蛋白体外补充实验，证明其可逆转神经细胞中过氧化物酶体功能缺陷，为相关脑部疾病的基因治疗提供潜在策略。
4. **标题**: *Optimization of ACBD5 Recombinant Expression in Insect Cells for Antibody Production*
**作者**: Chen D, et al.
**摘要**: 采用杆状病毒-昆虫细胞系统优化ACBD5重组蛋白表达条件，获得高产量蛋白用于制备特异性抗体，并验证其在免疫组化中的应用。
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**说明**：上述文献为示例性质，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实文献。ACBD5相关研究多聚焦于其参与过氧化物酶体功能、脂代谢调控及与疾病(如脑视网膜病变)的关联。
ACBD5 (Acyl-CoA-binding domain-containing protein 5) is a peroxisomal membrane protein belonging to the acyl-CoA-binding domain-containing protein family. It plays a critical role in maintaining peroxisome-ER membrane contact sites through interactions with vesicle-associated membrane protein-associated protein B (VAPB) on the endoplasmic reticulum. This interaction facilitates lipid exchange, particularly for very-long-chain fatty acids (VLCFAs), and supports peroxisomal metabolic functions. ACBD5 contains a conserved acyl-CoA-binding domain, enabling it to bind acyl-CoA esters, which are essential intermediates in lipid biosynthesis and β-oxidation.
Recombinant ACBD5 protein is engineered for in vitro studies to explore its structural and functional properties. Typically produced in expression systems like E. coli or mammalian cells, it is purified using affinity tags (e.g., His-tag) for applications in biochemical assays, protein interaction studies, or structural analysis. Research has linked ACBD5 mutations to peroxisomal disorders, including retinal dystrophy, developmental delays, and VLCFA accumulation, highlighting its role in lipid homeostasis.
Current studies focus on its involvement in organelle communication, lipid transport mechanisms, and potential therapeutic targets for peroxisome-related diseases. Recombinant ACBD5 serves as a tool to investigate these pathways and screen for compounds modulating its activity. Despite progress, unresolved questions remain about its full interactome, regulatory mechanisms, and precise role in disease pathogenesis.
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