| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCE3A |
| Uniprot No | Q5TA76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-89aa |
| 氨基酸序列 | MSCQQNQQQCQPPPKCPAKSPAQCLPPASSSCAPSSGGCGPSSERSCCLS HHRCRRSHRCRCQSSNSCDRGSGQQGGSSSCGHSSAGCC |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LCE3A重组蛋白的虚构参考文献示例(仅供格式参考，实际文献请通过学术数据库查询)：
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1. **标题**: *Expression and purification of recombinant LCE3A protein in Escherichia coli*
**作者**: Zhang Y, et al.
**摘要**: 研究利用大肠杆菌系统表达LCE3A重组蛋白，优化了诱导条件并通过亲和层析纯化，验证其可用于体外皮肤屏障功能研究。
2. **标题**: *LCE3A gene knockout exacerbates psoriasis-like inflammation in mice*
**作者**: Wang L, et al.
**摘要**: 通过构建LCE3A缺陷小鼠模型，发现LCE3A缺失导致皮肤屏障受损，加重咪喹莫特诱导的银屑病样炎症反应，提示其保护作用。
3. **标题**: *Structural characterization of LCE3A protein and its interaction with lipids*
**作者**: Gupta S, et al.
**摘要**: 采用X射线晶体学解析LCE3A蛋白结构，发现其C端结构域与角质层脂质结合，可能参与表皮防水屏障的形成。
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**注意**：以上内容为模拟示例，实际文献请通过PubMed、Google Scholar等平台搜索关键词“LCE3A recombinant protein”或结合具体研究方向筛选。
LCE3A (Late Cornified Envelope 3A) is a member of the LCE protein family, which plays a critical role in epidermal differentiation and skin barrier function. These proteins are encoded by genes clustered within the Epidermal Differentiation Complex (EDC) on chromosome 1q21. a region associated with skin homeostasis and inflammatory disorders. LCE3A is specifically expressed in the granular and cornified layers of the epidermis, contributing to the formation of the cornified envelope—a highly cross-linked protein-lipid structure essential for protecting against environmental stressors, pathogens, and water loss.
Research has linked LCE3A genetic deletions or polymorphisms to susceptibility to psoriasis, atopic dermatitis, and other skin conditions, highlighting its role in maintaining epidermal integrity. Dysregulation of LCE3A expression may impair skin barrier function, triggering inflammatory responses or abnormal keratinocyte differentiation.
Recombinant LCE3A protein is produced using biotechnological platforms (e.g., bacterial or mammalian expression systems) to enable functional studies. Its purification typically involves affinity chromatography and refolding steps to ensure structural fidelity. As a research tool, recombinant LCE3A aids in investigating protein-protein interactions, signaling pathways in keratinocytes, and mechanisms underlying skin diseases. It also serves as an antigen for antibody development or a potential therapeutic candidate for barrier repair. Challenges in working with recombinant LCE3A include optimizing solubility and post-translational modifications to mimic native protein behavior. Ongoing studies aim to clarify its precise molecular functions and therapeutic relevance in dermatology.
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