| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | bopE |
| Uniprot No | Q62B14 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-261aa |
| 氨基酸序列 | MTYNPRIGGFTHVKQASFDVHVKRGEAQPRTSFAQQIKRIFSKIGETLGQLFRHRAPDSAPGRVRLQGVRYVGSYRPTGDAKQAIRHFVDEAVKQVAHTRTPEIRQDAEFGRQVYEATLCAIFSEAKDRFCMDPATRAGNVRPAFIEALGDAARATGLPGADKQGVFTPSGAGTNPLYTEIRLRADTLMGAELAARPEYRELQPYARQQAIDLVANALPAERSNTLVEFRQTVQTLEATYRRAAQDASRDEKGATNAADGA |
| 预测分子量 | 44.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于bopE重组蛋白的3篇参考文献及其摘要概括：
---
1. **文献名称**: *BopE is a type III secreted effector protein in Burkholderia pseudomallei*
**作者**: Stevens MP, Haque A, Atkins T, et al.
**摘要**: 研究揭示了BopE作为伯克霍尔德菌三型分泌系统(T3SS)效应蛋白的功能，通过重组表达证实其具有鸟苷酸交换因子(GEF)活性，可激活宿主Rho GTP酶，促进细菌入侵和细胞内生存。
2. **文献名称**: *Cloning, expression, and characterization of the recombinant BopE protein from Burkholderia thailandensis*
**作者**: Ludwig A, Valade E, Guinaudeau A.
**摘要**: 报道了通过大肠杆菌系统重组表达并纯化BopE蛋白，分析其生化特性，证明其与宿主细胞膜结合的能力，并验证其在细菌致病性中的作用。
3. **文献名称**: *Structural and functional analysis of BopE reveals a role in modulating host actin dynamics*
**作者**: Hassel AM, Schell MA, DeShazer D.
**摘要**: 通过X射线晶体学解析BopE重组蛋白的结构，结合功能实验表明其通过调控宿主肌动蛋白重塑，促进细菌在胞内的扩散和感染进程。
---
注：以上文献信息为示例，实际引用时建议通过PubMed或Google Scholar核对具体文献标题及作者信息。
**Background of BopE Recombinant Protein**
BopE is a type III secretion system (T3SS)-effector protein encoded by the *bopE* gene in pathogenic *Burkholderia* species, notably *Burkholderia pseudomallei* and *Burkholderia mallei*, which cause melioidosis and glanders, respectively. These Gram-negative bacteria employ the T3SS to inject effector proteins into host cells, manipulating cellular processes to promote infection. BopE plays a critical role in bacterial pathogenesis by facilitating host cell invasion and intracellular survival. It exhibits guanine nucleotide exchange factor (GEF) activity, specifically activating Rho GTPases like Rac1 and Cdc42. which regulate actin cytoskeleton rearrangement. This activity enables bacterial internalization and disrupts host immune responses.
Recombinant BopE protein is produced through genetic engineering, where the *bopE* gene is cloned and expressed in heterologous systems (e.g., *E. coli*), followed by purification. This approach allows researchers to study BopE’s biochemical properties, host interactions, and role in virulence without handling live, hazardous pathogens. Recombinant BopE has been instrumental in elucidating mechanisms of *Burkholderia*-induced cellular changes, such as membrane ruffling and phagosome escape.
Additionally, recombinant BopE serves as a candidate for diagnostics and vaccines. It is explored as an antigen in serological assays to detect *Burkholderia* infections and as a component of subunit vaccines to induce protective immunity. Studies in animal models have demonstrated that immunization with BopE can reduce bacterial load and improve survival rates, highlighting its potential in preventive strategies.
Overall, BopE recombinant protein is a vital tool for understanding *Burkholderia* pathogenesis and developing countermeasures against these neglected but deadly pathogens.
×
