| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CLVS2 |
| Uniprot No | Q5SYC1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-327aa |
| 氨基酸序列 | MTHLQAGLSPETLEKARLELNENPDTLHQDIQEVRDMVITRPDIGFLRTDDAFILRFLRARKFHHFEAFRLLAQYFEYRQQNLDMFKSFKATDPGIKQALKDGFPGGLANLDHYGRKILVLFAANWDQSRYTLVDILRAILLSLEAMIEDPELQVNGFVLIIDWSNFTFKQASKLTPSMLRLAIEGLQDSFPARFGGIHFVNQPWYIHALYTVIRPFLKEKTRKRIFLHGNNLNSLHQLIHPEILPSEFGGMLPPYDMGTWARTLLDHEYDDDSEYNVDSYSMPVKEVEKELSPKSMKRSQSVVDPTVLKRMDKNEEENMQPLLSLD |
| 预测分子量 | 54.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CLVS2(Calsyntenin-2)重组蛋白的模拟参考文献示例(注：文献为虚构示例，仅作格式参考)：
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1. **文献名称**: "Expression and Functional Characterization of Recombinant CLVS2 in Neuronal Cell Models"
**作者**: Smith A, et al.
**期刊**: *Journal of Neurochemistry*
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化了重组CLVS2蛋白，验证了其在神经元细胞中对突触囊泡运输的调控作用，发现其通过结合Rab GTPases促进囊泡运输效率。
2. **文献名称**: "Structural Insights into CLVS2-Lipid Interactions Using Recombinant Protein"
**作者**: Zhang L, et al.
**期刊**: *Biophysical Journal*
**摘要**: 通过X射线晶体学解析了重组CLVS2的钙离子结合结构域，揭示了其与细胞膜磷脂酰丝氨酸的特异性结合机制，为CLVS2在膜运输中的功能提供结构基础。
3. **文献名称**: "CLVS2 Recombinant Protein Attenuates Amyloid-β Toxicity in Alzheimer’s Disease Models"
**作者**: Tanaka K, et al.
**期刊**: *Molecular Neurodegeneration*
**摘要**: 利用HEK293细胞表达的重组CLVS2蛋白，证明其可通过调控APP(淀粉样前体蛋白)加工减少Aβ寡聚体生成，在阿尔茨海默病小鼠模型中显示神经保护作用。
4. **文献名称**: "Development of a CLVS2 Knockout Cell Line and Rescue with Recombinant Protein"
**作者**: Müller S, et al.
**期刊**: *Cell Biology International*
**摘要**: 通过CRISPR技术构建CLVS2基因敲除细胞系，并利用重组CLVS2蛋白恢复其内体运输功能，证明CLVS2在维持溶酶体稳态中的必要性。
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如需真实文献，建议通过PubMed/Google Scholar检索关键词：
`"CLVS2 recombinant protein"` 或 `"Calsyntenin-2 expression"`。
**Background of CLVS2 Recombinant Protein**
CLVS2 (Clavesin-2), also known as COBL-like protein 2. is a conserved eukaryotic protein implicated in membrane trafficking and cellular organization. It shares structural homology with proteins involved in vesicle formation, such as the COG (Conserved Oligomeric Golgi) complex and Sec23/Sec24 cargo adaptors. CLVS2 is primarily localized to the Golgi apparatus and endosomal compartments, where it facilitates intracellular transport processes, including vesicle budding, cargo sorting, and membrane fusion.
Functionally, CLVS2 interacts with components of the coatomer complex and small GTPases like Arf1. suggesting its role in maintaining Golgi integrity and regulating secretory pathways. Studies highlight its involvement in autophagy, lysosomal biogenesis, and cell adhesion by modulating membrane dynamics. Dysregulation of CLVS2 has been linked to neurodegenerative disorders and cancer, where altered vesicular trafficking contributes to pathological protein aggregation or uncontrolled cell proliferation.
The recombinant CLVS2 protein is engineered for research applications, typically expressed in mammalian or insect cell systems to preserve post-translational modifications. Its purified form enables in vitro studies on membrane trafficking mechanisms, protein-protein interactions, and drug screening for diseases associated with secretory pathway defects. Recent work also explores CLVS2 as a potential biomarker or therapeutic target, emphasizing its critical yet understudied role in cellular homeostasis. Further research aims to unravel its precise molecular mechanisms and translational relevance.
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