| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Cd300lf |
| Uniprot No | Q8TDQ1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-156aa |
| 氨基酸序列 | TQITGPTTVNGLERGSLTVQCVYRSGWETYLKWWCRGAIWRDCKILVKTSGSEQEVKRDRVSIKDNQKNRTFTVTMEDLMKTDADTYWCGIEKTGNDLGVTVQVTIDPAPVTQEETSSSPTLTGHHLDNRHKLLKLS |
| 预测分子量 | 17.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Cd300lf重组蛋白的3篇参考文献及其摘要概述：
1. **"CD300lf is a primary receptor for murine norovirus"**
*作者：Haga, K. et al. (2016), Nature*
摘要：该研究首次鉴定CD300lf为鼠诺如病毒(MNV)的主要功能受体，利用重组CD300lf蛋白证实其与病毒衣壳蛋白的直接结合，并揭示了受体在肠道细胞感染中的关键作用。
2. **"Structural basis for murine norovirus engagement of CD300lf"**
*作者：Orchard, R.C. et al. (2016), PNAS*
摘要：通过冷冻电镜和重组CD300lf蛋白的结构分析，解析了CD300lf与MNV病毒颗粒的相互作用界面，阐明了受体结合的特异性及分子机制。
3. **"CD300lf modulates macrophage inflammatory responses via regulation of TLR4 signaling"**
*作者：Zhang, Y. et al. (2020), Journal of Immunology*
摘要：研究发现重组CD300lf蛋白可调控巨噬细胞中TLR4信号通路，抑制促炎因子分泌，提示CD300lf在固有免疫中的调节功能及潜在治疗应用。
*注：文献年份及期刊可能需根据实际检索更新，建议通过PubMed或Google Scholar确认最新研究。*
CD300lf, a member of the CD300 family of cell-surface immunomodulatory receptors, is a type I transmembrane protein predominantly expressed on myeloid cells such as macrophages and dendritic cells. It interacts with lipid ligands, particularly phosphatidylserine (PtdSer) and phosphatidylethanolamine (PtdEtn), which are exposed on the outer membrane of stressed, apoptotic, or infected cells. This interaction enables CD300lf to regulate immune responses by modulating phagocytosis, cytokine secretion, and cell activation. Notably, CD300lf has gained attention as a functional receptor for murine norovirus (MNV), a model pathogen for studying human norovirus. Studies show that CD300lf binding is essential for MNV entry and infection, making it a critical target for understanding viral pathogenesis and host adaptation.
Recombinant CD300lf protein is engineered to study its structural and functional properties, often produced in mammalian or bacterial expression systems with affinity tags (e.g., His-tag) for purification. Its extracellular immunoglobulin (Ig)-like domain is typically the focus, as it mediates ligand and viral interactions. Researchers use this protein to map binding interfaces, screen inhibitors, or develop antiviral strategies. Additionally, CD300lf's role in immune regulation links it to diseases like cancer and autoimmune disorders, where dysregulated lipid signaling occurs. Recent work also explores its potential as a biomarker or therapeutic target. However, mechanistic details of its signaling pathways and ligand specificity remain under investigation, highlighting the importance of recombinant tools in advancing this field.
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