| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Zc3h12a |
| Uniprot No | Q5D1E8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-599aa |
| 氨基酸序列 | MSGPCGEKPVLEASPTMSLWEFEDSHSRQGTPRPGQELAAEEASALELQMKVDFFRKLGYSSTEIHSVLQKLGVQADTNTVLGELVKHGTATERERQTSPDPCPQLPLVPRGGGTPKAPNLEPPLPEEEKEGSDLRPVVIDGSNVAMSHGNKEVFSCRGILLAVNWFLERGHTDITVFVPSWRKEQPRPDVPITDQHILRELEKKKILVFTPSRRVGGKRVVCYDDRFIVKLAYESDGIVVSNDTYRDLQGERQEWKRFIEERLLMYSFVNDKFMPPDDPLGRHGPSLDNFLRKKPLTLEHRKQPCPYGRKCTYGIKCRFFHPERPSCPQRSVADELRANALLSPPRAPSKDKNGRRPSPSSQSSSLLTESEQCSLDGKKLGAQASPGSRQEGLTQTYAPSGRSLAPSGGSGSSFGPTDWLPQTLDSLPYVSQDCLDSGIGSLESQMSELWGVRGGGPGEPGPPRAPYTGYSPYGSELPATAAFSAFGRAMGAGHFSVPADYPPAPPAFPPREYWSEPYPLPPPTSVLQEPPVQSPGAGRSPWGRAGSLAKEQASVYTKLCGVFPPHLVEAVMGRFPQLLDPQQLAAEILSYKSQHPSE |
| 预测分子量 | 69.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"MCPIP1 regulates TLR3/4- and RIG-I-induced IFN-β expression and type I IFN response"**
*Authors: Mizgalska D, et al.*
摘要：研究报道Zc3h12a(MCPIP1)重组蛋白通过其RNA酶活性调控TLR3/4和RIG-I信号通路，抑制炎症因子(如IL-6、IL-12)及I型干扰素(IFN-β)的过度表达，维持免疫稳态。
2. **"Structural and functional analysis of the MCPIP1 protein in RNA degradation"**
*Authors: Suzuki HI, et al.*
摘要：通过重组Zc3h12a蛋白的晶体结构解析，揭示其CCCH锌指结构域与RNA酶活性区域协同作用，介导炎症相关mRNA(如IL-6、IL-1β)的降解，并参与miRNA前体加工。
3. **"MCPIP1 inhibits hepatocellular carcinoma progression by targeting AURKA mRNA"**
*Authors: Liang J, et al.*
摘要：实验证明重组Zc3h12a蛋白通过降解致癌基因AURKA的mRNA，抑制肝癌细胞增殖并诱导凋亡，为靶向Zc3h12a的癌症治疗提供理论基础。
4. **"Zc3h12a controls adaptive immune responses by targeting IL-2 mRNA"**
*Authors: Iwasaki H, et al.*
摘要：研究显示重组Zc3h12a蛋白通过结合并降解T细胞中IL-2 mRNA，调控T细胞活化与分化，影响自身免疫疾病及移植排斥反应的病理进程。
Zc3h12a, also known as MCPIP1 (monocyte chemotactic protein-induced protein 1), is a multifunctional protein encoded by the Zc3h12a gene. Initially identified as a critical regulator of immune homeostasis, it plays a pivotal role in modulating inflammatory responses and cellular processes. Structurally, Zc3h12a contains a conserved CCCH-type zinc finger domain and a PilT N-terminal (PIN)-like RNase domain, enabling its RNA-binding and endoribonuclease activities. These features allow it to degrade specific mRNAs, including pro-inflammatory cytokines such as IL-6 and IL-12. thereby controlling their expression post-transcriptionally. Additionally, Zc3h12a interacts with signaling pathways like NF-κB and AP-1. further fine-tuning immune activation and preventing excessive inflammation.
Beyond its anti-inflammatory role, Zc3h12a is involved in autophagy, apoptosis, and cell differentiation. Its dysfunction has been linked to autoimmune diseases, atherosclerosis, metabolic disorders, and cancer, highlighting its broad physiological relevance. For instance, Zc3h12a-deficient mice develop severe autoimmune phenotypes, underscoring its necessity in maintaining immune tolerance.
Recombinant Zc3h12a protein is engineered using expression systems (e.g., E. coli or mammalian cells) to produce purified, bioactive forms for research and therapeutic exploration. It serves as a tool to study molecular mechanisms of RNA degradation, immune regulation, and disease pathogenesis. In drug development, recombinant Zc3h12a is investigated for its potential to suppress aberrant inflammation or target disease-associated mRNAs. However, challenges remain in optimizing its stability, delivery, and specificity for clinical applications. Ongoing research continues to unravel its complex roles, positioning Zc3h12a as a promising target for modulating immune-related disorders and beyond.
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